ISOLATION AND CHARACTERIZATION OF PC-3 HUMAN PROSTATIC TUMOR SUBLINES WHICH PREFERENTIALLY METASTASIZE TO SELECT ORGANS IN SCID MICE
ISOLATION AND CHARACTERIZATION OF PC-3 HUMAN PROSTATIC TUMOR SUBLINES WHICH PREFERENTIALLY METASTASIZE TO SELECT ORGANS IN SCID MICE
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DOI：
10.1111/j.1432-0436.1991.tb00250.x
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发表时间：
1991-11-01
期刊：
影响因子：
2.9
通讯作者：
STEARNS, ME
中科院分区：
文献类型：
--
作者：
WANG, M;STEARNS, ME
我们已经开发并部分表征了一个小鼠模型系统来研究人类前列腺肿瘤细胞在体内的转移。为了开发这个模型，我们选择了高侵入性（3 x i）和非侵入性（3 x i）。在Boyden室趋化试验中，PC-3人前列腺肿瘤亚群基于跨重构基底膜屏障（Matrigel）迁移能力的增强和减弱。当3 × i细胞静脉注射到严重联合免疫缺陷（scid）小鼠的尾静脉时，细胞最初转移到多种组织，通过使用[I-125] IUdR标记的细胞和组织学证实了这一点。最终分离出四种不同的亚群，它们优先转移到腰椎（PC-3 ML）、右脸颊下颌区域（PC-3 MC）、肋骨软骨（PC-3 MR）和右前膝骨（PC-3 MK），转移率几乎等于80%。不同部位的移植实验表明，器官转移可能通过宿主组织以某种方式传递给肿瘤亚克隆。
We have developed and partially characterized a mouse model system for studying human prostate tumor cell metastases in vivo. To develop this model we have selected highly invasive (3 x I.) and non-invasive (3 x N.I.) PC-3 human prostatic tumor sublines based on enhanced and diminished capacities to migrate across a reconstituted basement membrane barrier (Matrigel) in Boyden chamber chemotactic assays. When the 3 x I. cells were injected intravenously (i.v.) in the tail vein of severe combined immune deficient (scid) mice, the cells initially metastasized to a wide variety of tissues as demonstrated by using [I-125] IUdR labeled cells and histology. Four distinct sublines were eventually isolated which preferentially metastasized at almost-equal-to 80% efficiency to the lumbar vertebrae (PC-3 ML), the mandibular region of the right cheek (PC-3 MC), the rib cartilage (PC-3 MR), and the right front knee bone (PC-3 MK), respectively. Implantation experiments at different sites indicated that organ metastases may somehow be conferred on the tumor subclones by the host tissue.