Progressive polarization towards a T helper/cytotoxic type‐1 cytokine pattern during age‐dependent maturation of the immune response inversely correlates with CD30 cell expression and serum concentration
Progressive polarization towards a T helper/cytotoxic type‐1 cytokine pattern during age‐dependent maturation of the immune response inversely correlates with CD30 cell expression and serum concentration
复制标题
在免疫反应的年龄依赖性成熟过程中，逐渐向 T 辅助细胞/细胞毒性 1 型细胞因子模式极化，与 CD30 细胞表达和血清浓度呈负相关
DOI：
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发表时间：
1999
影响因子：
4.6
通讯作者：
Pizzolo
中科院分区：
文献类型：
--
作者：
Krampera;Vinante;Tavecchia;Morosato;Chilosi;Romagnani;Zanolin;Pizzolo
为了研究免疫应答年龄依赖性成熟过程中T细胞细胞因子的特征，我们通过流式细胞术单细胞分析法评估了从脐带血到成年期不同年龄组正常个体体外非特异性刺激后CD 4+和CD 8+循环细胞的细胞因子表达。此外，我们将这些淋巴细胞细胞因子模式与CD 30的表达/释放相关联，CD 30是肿瘤坏死因子（TNF）受体超家族的成员，已被认为与T辅助细胞/细胞毒性（Th（c））2型免疫应答相关，以在体内非病理条件下验证这种关联。结果显示，随着年龄的增长，循环Th（c）1型，干扰素γ（IFN-γ）和/或IL-2产生T细胞逐渐增加，相反，纳塔尔后组中CD 4 +/IL-4+ T细胞的数量稳定，尽管高于脐带血样本。此外，血清可溶性CD 30（sCD 30）水平和循环CD 4 +/CD 30+和CD 8 +/CD 30 + T细胞的数量显着高于5岁以下的儿童相比，无论是在脐带血或血液中发现的年龄较大的儿童和成人。 这些数据支持在免疫应答的年龄依赖性成熟过程中Th（c）细胞因子谱向Th（c）1模式进行性极化的概念。此外，在Th（c）1转变发生之前的婴儿早期，CD 30表达/释放达到峰值，尽管与循环IL-4+ T细胞的显著增加无关，但提出了CD 30和Th（c）2型免疫应答之间可能存在体内关系的问题。
In order to investigate the T cell cytokine profile during age‐dependent maturation of the immune response, we evaluated the cytokine expression of CD4+ and CD8+ circulating cells by flow cytometric single‐cell analysis after non‐specific stimulation in vitro in different age groups of normal individuals, from cord blood to adulthood. Moreover, we correlated these lymphocyte cytokine patterns with the expression/release of CD30, a member of the tumour necrosis factor (TNF) receptor superfamily, which has been suggested to be related to the T helper/cytotoxic (Th(c))2‐type immune responses, in order to verify this association in vivo, in non‐pathological conditions. The results showed a progressive increase of circulating Th(c)1‐type, interferon‐gamma (IFN‐γ)‐ and/or IL‐2‐producing T cells along with ageing and, conversely, a stable number, although higher than in cord blood samples, of CD4+/IL‐4+ T cells in the post‐natal groups. In addition, serum levels of soluble CD30 (sCD30) and numbers of circulating CD4+/CD30+ and CD8+/CD30+ T cells were significantly higher in children aged < 5 years in comparison with those found either in cord blood or in blood from both older children and adults. These data support the concept of a progressive polarization of the Th(c) cell cytokine profile towards the Th(c)1 pattern during age‐dependent maturation of the immune response. Moreover, the peak of CD30 expression/release in early infancy before the Th(c)1 shifting occurs, although not associated with a significant increase of circulating IL‐4+ T cells, raises the question of the possible relationship in vivo between CD30 and Th(c)2‐type immune responses.
影响因子：
20.3
作者：
PICKER, LJ;SINGH, MK;MAINO, VC
通讯作者：
MAINO, VC