CULTURING AND ROBOTIC HARVESTING OF BONE-MARROW, LYMPH-NODES, PERIPHERAL-BLOOD, FIBROBLASTS, AND SOLID TUMORS WITH INSITU TECHNIQUES
CULTURING AND ROBOTIC HARVESTING OF BONE-MARROW, LYMPH-NODES, PERIPHERAL-BLOOD, FIBROBLASTS, AND SOLID TUMORS WITH INSITU TECHNIQUES
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DOI：
10.1016/0165-4608(88)90312-3
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发表时间：
1988-05-01
影响因子：
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通讯作者：
DEWALD, GW
中科院分区：
文献类型：
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作者：
SPURBECK, JL;CARLSON, RO;DEWALD, GW
我们已经修改了我们的原位方法培养造血细胞，并已成功地使用这种新的程序培养细胞从骨髓，淋巴结，外周血，成纤维细胞，和实体瘤。我们对这种方法非常满意，在过去的2.5年里，我们一直在日常实践中使用它。原位方法的一个方便的特征是可以用市售的机器人收获系统（Modified Tecan Robotic SampleProcessor Model 505）处理培养物。该系统也适用于羊水的原位培养。原位培养物的机器人收获提供了一致和准确的程序，每个案例节省了约10-15分钟的技术人员时间。
We have modified our in situ method for culturing amniocytes and have successfully used this new procedure to culture cells from bone marrow, lymph nodes, peripheral blood, fibroblasts, and solid tumors. We have been so satisfied with this method that we have used it in our routine practice for the past 2.5 years. A convenient feature of the in situ method is that the cultures can be processed with a commercially available robotic harvesting system (Modified Tecan Robotic SampleProcessor Model 505). This system also works well for in situ cultures of amniotic fluids. Robotic harvesting of in situ cultures provides a consistent and accurate procedure that saves about 10-15 minutes of technologist time per case.