IMMUNOHISTOCHEMICAL STUDY OF TOPOISOMERASE II-ALPHA EXPRESSION IN PRIMARY DUCTAL CARCINOMA OF THE BREAST
IMMUNOHISTOCHEMICAL STUDY OF TOPOISOMERASE II-ALPHA EXPRESSION IN PRIMARY DUCTAL CARCINOMA OF THE BREAST
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DOI：
10.1136/jcp.48.2.147
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发表时间：
1995-02-01
影响因子：
3.4
通讯作者：
VANMARCK, E
中科院分区：
文献类型：
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作者：
HELLEMANS, P;VANDAM, PA;VANMARCK, E
目的-研究拓扑异构酶II-α在原发性浸润性导管乳腺癌中的表达模式；方法-对 63 个原发性浸润性导管乳腺癌的冷冻切片进行拓扑异构酶 II-α 免疫组织化学染色。通过计数不同随机视野中的至少 500 个细胞来量化核免疫反应性，结果以拓扑异构酶 II-α 染色呈阳性的细胞百分比表示。结果 - 在具有高度可变的肿瘤间和肿瘤内核反应性的所有谣言中检测到拓扑异构酶 II-α 核免疫反应性（细胞核中位数 14%；范围 2-62%）。较高水平的拓扑异构酶 II-α 表达与较高的肿瘤分级、较大的肿瘤大小、淋巴结状态以及诊断时是否存在远处转移密切相关。未发现与绝经状态、类固醇激素受体状态、无病生存或总生存相关。结论-拓扑异构酶 II-α 的表达与不良预后因素的存在相关。乳腺癌中拓扑异构酶 II-α 表达的免疫组织化学评估可能有助于调整拓扑异构酶 II 抑制剂的化疗。
Aims-To study the patterns of expression of topoisomerase II-alpha in primary invasive ductal breast carcinomas; to correlate this expression with clinicopathological data and prognosis.Methods-Cryostat sections from 63 primary invasive ductal breast carcinomas were stained immunohistochemically for topoisomerase II-alpha. Nuclear immunoreactivity was quantified by counting at least 500 cells in different random fields and results were expressed as per cent of cells staining positively for topoisomerase II-alpha.Results-Topoisomerase II-alpha nuclear immunoreactivity (median 14% of nuclei; range 2-62%) was detected in all rumours with highly variable intertumour and intratumour nuclear reactivity. Higher levels of topoisomerase II-alpha expression were strongly related to higher tumour grade, larger tumour size, nodal status, and the presence of distant metastases at diagnosis. No correlation was found with menopausal status, steroid hormone receptor status, disease free survival, or overall survival.Conclusions-Expression of topoisomerase II-alpha is related to the presence of poor prognostic factors. Immunohistochemical assessment of topoisomerase II-alpha expression in breast cancer could be potentially useful for tailoring chemotherapy with topoisomerase II inhibitors.