Corrositex®, BCOP and HET-CAM as alternative methods to animal experimentation
Corrositex®, BCOP and HET-CAM as alternative methods to animal experimentation
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DOI：
10.1590/s1984-82502009000400021
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发表时间：
2009-12-01
影响因子：
1.3
通讯作者：
Salgado, Hérida Regina Nunes
中科院分区：
文献类型：
--
作者：
Cazedey, Edith Cristina Laignier;Carvalho, Flávia Chiva;Salgado, Hérida Regina Nunes
动物试验被用作毒理学和调查研究的模型。然而，这种测试被认为是不人道的，因为它们会给实验动物带来疼痛和痛苦，而这些方法往往是主观的。要求保护动物的抗议者质疑体内试验的有效性，并建议引入替代的体外方法。监管和开发新的替代动物模型的国际组织，如替代方法验证机构间协调委员会（ICCVAM）、美国国立卫生研究院（NIH）、经济合作与发展组织（OECD），已经表示进行初步测定和执行顺序试验，这些试验考虑了体外测定的理化性质和数据，在进行体内研究之前。在此背景下，本文的目的是选择有前途的替代方法，如Corrositex（R），BCOP和HET-CAM，旨在改善或取代动物的使用，减少他们的痛苦。
Tests in animals are used as models in toxicological and investigative studies. However, such tests have been considered inhumane because they can cause pain and suffering to experimental animals, while these methods can often be subjective. Protests calling for animal protection have questioned the effectiveness of in vivo tests and suggest the introduction of alternative, in vitro methods. International organizations, such as the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), the National Institute of Health (NIH), the Organization for Economic Co-operation and Development (OECD), that regulate and develop new alternative animal models, have indicated the running of preliminary assays and execution of sequential tests, which consider physical-chemical properties and data of in vitro assays, before performing in vivo studies. Towards this background, the objective of the present article was to select promising alternative methods such as Corrositex (R), BCOP and HET-CAM, intended to refine or replace the use of animals and reduce their suffering.