Human matrix attachment regions insulate transgene expression from chromosomal position effects in Drosophila melanogaster
Human matrix attachment regions insulate transgene expression from chromosomal position effects in Drosophila melanogaster
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DOI：
10.1128/mcb.18.4.2382
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发表时间：
1998-04-01
影响因子：
5.3
通讯作者：
Fournier, REK
中科院分区：
文献类型：
--
作者：
Namciu, SJ;Blochlinger, KB;Fournier, REK
用含有白色表达盒的p元件载体对白色（-）果蝇胚胎进行种系转化，由于转基因插入位点的位置效应导致果蝇具有不同的颜色表型，这些位置效应可以通过在体内具有绝缘体活性的特定DNA元件（如果蝇scs和scs’元件）来治愈。我们已经使用该系统来确定人体基质附着区（MARs）是否可以在体内作为绝缘体元件。在黑胃果蝇中，载脂蛋白B和α 1-抗胰蛋白酶两个不同的人类基因座将白色转基因的表达与位置效应隔离，这两个元素都降低了转基因表达的变异性，而不增加白色基因的表达水平。相比之下，含有人类DNA片段而不具有基质结合活性的白色转基因在果蝇转化体中的表达变化很大。这些数据表明，人类的火星在体内可以作为绝缘体元件。
Germline transformation of white(-) Drosophila embryos with P-element vectors containing white expression cassettes results in flies with different eve color phenotypes due to position effects at the sites off transgene insertion, These position effects can be cured by specific DNA elements, such as the Drosophila scs and scs' elements, that have insulator activity in vivo. We have used this system to determine whether human matrix attachment regions (MARs) can function as insulator elements in vivo. Two different human MARs, from the apolipoprotein B and alpha 1-antitrypsin loci, insulated white transgene expression from position effects in Drosophila melanogaster, Both elements reduced variability in transgene expression without enhancing levels of white gene expression. In contrast, expression of white transgenes containing human DNA segments without matrix-binding activity was highly variable in Drosophila transformants. These data indicate that human MARs can function as insulator elements in vivo.