MECHANISM OF CITRININ-INDUCED DYSFUNCTION OF MITOCHONDRIA .1. EFFECTS ON RESPIRATION, ENZYME-ACTIVITIES AND MEMBRANE-POTENTIAL OF RENAL CORTICAL MITOCHONDRIA
MECHANISM OF CITRININ-INDUCED DYSFUNCTION OF MITOCHONDRIA .1. EFFECTS ON RESPIRATION, ENZYME-ACTIVITIES AND MEMBRANE-POTENTIAL OF RENAL CORTICAL MITOCHONDRIA
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DOI：
10.1002/jat.2550120209
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发表时间：
1992-04-01
影响因子：
3.3
通讯作者：
KLUPPEL, LW
中科院分区：
文献类型：
--
作者：
CHAGAS, GM;CAMPELLO, AP;KLUPPEL, LW
柠檬酸苷降低了大鼠肾皮质线粒体的磷酸化效率，这可以通过降低呼吸控制系数（RC）和ADP/O比值来推测。线粒体产生的跨膜电位（DELTA-psi）和添加ADP后的去极化也降低。Citrinin （1.0 mM）抑制几乎所有与呼吸链相关的酶，并增加琥珀酸细胞色素c还原酶和琥珀酸氧化酶（偶联）的活性。苹果酸和谷氨酸脱氢酶也受到抑制。柠檬酸苷对磷酸化效率的抑制作用可能与以下发现有关：对配合物I的影响；对ATP合成酶复合物的作用；跨膜电位的部分抑制。
Citrinin depresses the phosphorylation efficiency of rat renal cortical mitochondria, as inferred from the decrease of the respiratory control coefficient (RC) and ADP/O ratios. The transmembrane potential (DELTA-psi) developed by energized mitochondria and the depolarization upon ADP addition are also decreased. Citrinin (1.0 mM) inhibits almost all enzymes linked to the respiratory chain and increases the activity of succinate cytochrome c reductase and succinate oxidase (coupled). Malate and glutamate dehydrogenases are also inhibited. The inhibitory action of citrinin on phosphorylation efficiency could be related to the following findings: the effect on complex I; the action on the ATP synthetase complex; the partial inhibition of the transmembrane potential.