SPECIFIC MONOCLONAL IGM IS A POTENT ADJUVANT IN MURINE MALARIA VACCINATION
SPECIFIC MONOCLONAL IGM IS A POTENT ADJUVANT IN MURINE MALARIA VACCINATION
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DOI：
10.1038/302256a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
PLAYFAIR, JHL
中科院分区：
文献类型：
--
作者：
HARTE, PG;COOKE, A;PLAYFAIR, JHL
最近在小鼠系统中进行的实验1，2表明，子代被动获得母体抗疟疾IgG抗体，同时赋予一定程度的抗原发感染免疫力，矛盾的是，通过疫苗接种阻止了获得性免疫的产生。因此，鉴于早期发现3关于特异性IgM和IgG抗体的竞争效应，我们研究了特异性单克隆IgM抗体是否可用于增强对血液期鼠疟疾疫苗的反应。我们现在报告，少量的纯化的单克隆抗寄生虫IgM可以特异性地增强免疫接种后的启动和记忆细胞的产生，感染后的存活率证明了这一点，并且这种效果的大小大于更传统的非特异性佐剂（百日咳博德特氏菌）。此外，在免疫母体的后代中，由于母体IgG的存在，疫苗接种无效长达8周1，2，我们发现IgM与疫苗一起施用时可以通过其佐剂作用完全克服这种抑制。
Recent experiments in the murine system1,2have indicated that the passive acquisition by offspring of maternal anti-malarial IgG antibodies while conferring some degree of immunity against a primary infection, paradoxically prevents the generation of acquired immunity through vaccination. Therefore, in view of earlier findings3concerning the competitive effects of specific IgM and IgG antibodies, we investigated whether specific monoclonal IgM antibodies could be used to potentiate the response to a blood-stage murine malaria vaccine. We now report that small amounts of purified monoclonal anti-parasite IgM can specifically potentiate both priming and memory cell generation in response to vaccination as evidenced by survival after infection, and that the magnitude of this effect is greater than that found with a more conventional nonspecific adjuvant (Bordetella pertussis). Additionally, in offspring of immune mothers, where vaccination is ineffective for up to 8 weeks due to the presence of maternal IgG1,2, we have found that IgM when administered with the vaccine can completely overcome this inhibition by its adjuvant effect.