Rational substrate and enzyme engineering of transketolase for aromatics
Rational substrate and enzyme engineering of transketolase for aromatics
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DOI：
10.1039/c2ob25751c
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发表时间：
2012-01-01
影响因子：
3.2
通讯作者：
Dalby, Paul A.
中科院分区：
文献类型：
--
作者：
Payongsri, Panwajee;Steadman, David;Dalby, Paul A.
用3-甲酰基苯甲酸和4-甲酰基苯甲酸作为分子探针，以及以前和新的转酮醇酶突变体，揭示了转酮醇酶与芳香醛反应速度的影响因素。与苯甲醛相比，D469T-TK以15-30倍的产率和250倍的比活性生产了新型的α，α-二羟基酮。
The uses of 3-formylbenzoic acid and 4-formylbenzoic acid as molecular probes along with previous and new transketolase mutants revealed the factors governing the rate of reaction between transketolase and aromatic aldehydes. The novel alpha,alpha-dihydroxyketones were produced at 15 to 30-fold higher yields and up to 250-fold higher specific activities with D469T TK when compared to those obtained for benzaldehyde.