Rapid Small‐Scale Preparation Method of Cell Surface Polysaccharides
Rapid Small‐Scale Preparation Method of Cell Surface Polysaccharides
复制标题
细胞表面多糖的快速小规模制备方法
作者：
T. Sugiyama;N. Kido;Y. Arakawa;M. Mori;S. Naito;M. Ohta;N. Kato
开发了一种快速小规模提取脂多糖（LPS）和荚膜多糖的方法，用于鉴定LPS的化学型和荚膜抗原的血清型。在不到2小时内从1.5 ml培养过夜的菌落的肉汤或悬浮液中制备细胞表面多糖。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析制备物的LPS，并通过双扩散凝胶沉淀（Ouchterlony）试验和硝酸纤维素膜印迹分析荚膜多糖。通过该方法获得的制剂的分析可以提供能够鉴定LPS化学型和芙膜抗原血清型的足够结果。
A rapid small‐scale method of extraction of lipopolysaccharide (LPS) and capsular polysaccharides was developed for the purpose of identification of chemotypes of LPS and serotypes of capsular antigens. Cell surface polysaccharides were prepared within less than 2 hr from 1.5 ml of broth or suspension of colonies cultured overnight. The preparations were analyzed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis for LPS, and by double diffusiori gel precipitation (Ouchterlony) test and blotting to nitrocellulose membrane for capsular polysaccharide. The analyses with the preparations obtained by the method could provide adequate results capable of identifying chemotypes of LPS and serotypes of capsular antigens.