Thrombospondin 2 expression is correlated with inhibition of angiogenesis and metastasis of colon cancer.
Thrombospondin 2 expression is correlated with inhibition of angiogenesis and metastasis of colon cancer.
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DOI：
10.1038/sj.bjc.6690056
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发表时间：
1999-01
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
血小板反应蛋白的两种亚型（TSP-1 和 TSP-2）在体外对血管生成具有抑制作用，尽管这些 TSP 亚型的生物学意义尚未在体内确定。我们通过逆转录聚合酶链反应 (RT-PCR) 分析检查了 61 例结肠癌中的 TSP-1 和 TSP-2 基因表达。这 61 例结肠癌中有 38 例 TSP-2 表达呈阳性，且肝转移发生率显着低于不表达 TSP-2 的结肠癌（P = 0.02）。 TSP-2 表达与这些结肠癌的 M0 分期显着相关（P = 0.03），而 TSP-1 表达与这些因素没有明显相关性。表达 TSP-2 的结肠癌患者的肝转移频率显着较低，与细胞相关的血管内皮生长因子 (VEGF-189) 亚型相关 (P = 0.0006)。通过 CD34 染色评估血管分布，TSP-2(–)/VEGF-189(+) 结肠癌显示基质中的血管计数和密度显着增加 (P < 0.0001)。与TSP-2(+)/VEGF-189(-)患者相比，TSP-2(–)/VEGF-189(+)结肠癌患者的预后也明显较差(P = 0.0014)。这些结果表明，结肠癌转移关键取决于血管生成，该血管生成是由血管抑制因子TSP-2和血管生成因子VEGF-189之间的平衡引起的。 © 1999 癌症研究运动
Two subtypes of thrombospondin (TSP-1 and TSP-2) have inhibitory roles in angiogenesis in vitro, although the biological significance of these TSP isoforms has not been determined in vivo. We examined TSP-1 and TSP-2 gene expression by reverse transcription polymerase chain reaction (RT-PCR) analysis in 61 colon cancers. Thirty-eight of these 61 colon cancers were positive for TSP-2 expression and showed hepatic metastasis at a significantly lower incidence than those without TSP-2 expression (P = 0.02). TSP-2 expression was significantly associated with M0 stage in these colon cancers (P = 0.03), whereas TSP-1 expression showed no apparent correlation with these factors. The colon cancer patients with TSP-2 expression showed a significantly low frequency of liver metastasis correlated with the cell-associated isoform of vascular endothelial growth factor (VEGF-189) (P = 0.0006). Vascularity was estimated by CD34 staining, and TSP-2(–)/VEGF-189(+) colon cancers showed significantly increased vessel counts and density in the stroma (P < 0.0001). TSP-2(–)/VEGF-189(+) colon cancer patients also showed significantly poorer prognosis compared with those with TSP-2(+) / VEGF-189(–) (P = 0.0014). These results suggest that colon cancer metastasis is critically determined by angiogenesis resulting from the balance between the angioinhibitory factor TSP-2 and angiogenic factor VEGF-189. © 1999 Cancer Research Campaign
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DOI：
10.1073/pnas.80.4.998
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
JAFFE, EA;RUGGIERO, JT;MOSHER, DF
通讯作者：
MOSHER, DF
影响因子：
5.3
作者：
LAIHO, M;RONNSTRAND, L;MASSAGUE, J
通讯作者：
MASSAGUE, J
DOI：
10.1016/s0022-2143(97)90141-4
发表时间：
1997-02-01
期刊：
JOURNAL OF LABORATORY AND CLINICAL MEDICINE
影响因子：
--
作者：
Panetti, TS;Chen, H;Mosher, DF
通讯作者：
Mosher, DF
影响因子：
3.2
作者：
Grossfeld, GD;Shi, SR;Cote, RJ
通讯作者：
Cote, RJ
DOI：
10.1073/pnas.88.11.5026
发表时间：
1991-06-01
影响因子：
11.1
作者：
IRUELAARISPE, ML;BORNSTEIN, P;SAGE, H
通讯作者：
SAGE, H