Physiological roles of semenogelin I and zinc in sperm motility and semen coagulation on ejaculation in humans
Physiological roles of semenogelin I and zinc in sperm motility and semen coagulation on ejaculation in humans
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DOI：
10.1093/molehr/gan003
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发表时间：
2008-03-01
影响因子：
4
通讯作者：
Morisawa, Masaaki
中科院分区：
文献类型：
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作者：
Yoshida, Kaoru;Kawano, Natsuko;Morisawa, Masaaki
在射精时，人类精子被认为是被机械地捕获，并通过与来自精囊的精液凝胶（Sgs）和来自前列腺的锌离子结合而在精液凝固物中变得不动。然而，蛋白质和重金属对精子活力的生理联合作用尚不清楚。在这里，我们已经首次证明，单独的Sg I，它不形成精液凝固物没有锌，是一个完整的人精子的活力在生理浓度的抑制剂。另一方面，单独的锌离子对精子活力没有影响，但使被浓度等于或小于1 mg/ml的Sg I抑制的精子活力恢复。这些观察结果表明，Sg I和锌对精子活力的作用不是机械的，而是生理的。石英晶体微天平分析表明，精子提取物首先结合到Sg I，然后锌离子，随后增加蛋白质的积累，这表明Sgs通过直接结合到精子表面来抑制精子活力。锌离子介导的Sg I的进一步积累可能在射精时捕获静止的精子。
At ejaculation, human sperm are considered to be mechanically trapped and become immotile in the semen coagulum by binding to semenogelins (Sgs) from the seminal vesicle and zinc ions from the prostate. However, the physiological combined roles of the protein and heavy metal on sperm motility are unknown. Here, we have first demonstrated that Sg I alone, which does not form the semen coagulum without zinc, is an inhibitor of the motility of intact human sperm at physiological concentration. On the other hand, zinc ions alone had no effect on sperm motility, but confer recovery of sperm motility that has been inhibited by Sg I at a concentration equal to or less than 1 mg/ml. These observations suggest that the roles played by Sg I and zinc on sperm motility are not mechanical but physiological. Quartz crystal microbalance analysis suggests that the sperm extract first bind to Sg I and then zinc ions which subsequently increase the protein accumulation, suggesting that Sgs inhibit sperm motility by directly binding to the sperm surface. Further accumulation of Sg I mediated by zinc ions may entrap the quiescent sperm at semen ejaculation.