MicroRNA-144 acts as a tumor suppressor by targeting Rho-associated coiled-coil containing protein kinase 1 in osteosarcoma cells (Retracted article. See vol. 24, pg. 705, 2021)
MicroRNA-144 acts as a tumor suppressor by targeting Rho-associated coiled-coil containing protein kinase 1 in osteosarcoma cells (Retracted article. See vol. 24, pg. 705, 2021)
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DOI：
10.3892/mmr.2015.3937
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发表时间：
2015-09-01
影响因子：
3.4
通讯作者：
Liu, Guohong
中科院分区：
文献类型：
--
作者：
Huang, Jianjun;Shi, Ying;Liu, Guohong
microRNA（miRs）已被证明与人类恶性肿瘤的发生和进展中的多个过程相关。先前的研究已经观察到miR-144在几种类型的癌症中的异常下调，包括骨肉瘤。然而，miR-144的功能及其在骨肉瘤中的潜在机制仍有待阐明。本研究表明，与正常对照相比，miR-144在骨肉瘤组织和细胞系中显著下调。miR-144的恢复显著抑制MG-63骨肉瘤细胞的增殖、迁移和侵袭。此外，Rho相关卷曲螺旋蛋白激酶1（ROCK 1）被鉴定为MG-63骨肉瘤细胞中miR-144的新靶点。此外，ROCK 1的敲低抑制MG-63骨肉瘤细胞的增殖、迁移和侵袭，其程度与miR-144过表达的影响相似。此外，ROCK 1 mRNA在骨肉瘤组织中表达增强，并与miR-144表达呈负相关。总之，本研究的结果表明，miR-144通过靶向ROCK 1在骨肉瘤中起肿瘤抑制剂的作用。
MicroRNAs (miRs) have been demonstrated to be associated with multiple processes in the development and progression of human malignancies. Previous studies have observed aberrant downregulation of miR-144 in several types of cancer, including osteosarcoma. However, the function of miR-144 and the underlying mechanism in osteosarcoma remain to be elucidated. The present study indicated that miR-144 was markedly downregulated in osteosarcoma tissues and cell lines compared with that in the normal controls. Restoration of miR-144 significantly inhibited cell proliferation, migration and invasion of MG-63 osteosarcoma cells. In addition, Rho-associated coiled-coil containing protein kinase 1 (ROCK1) was identified as a novel target of miR-144 in MG-63 osteosarcoma cells. Furthermore, knockdown of ROCK1 suppressed the proliferation, migration and invasion of MG-63 osteosarcoma cells to a similar extent to the effects of miR-144 overexpression. In addition, the mRNA expression of ROCK1 was increased in osteosarcoma tissues and was negatively correlated with the expression of miR-144. In conclusion, the results of the present study suggested that miR-144 acts as a tumor suppressor by targeting ROCK1 in osteosarcoma.