Cell-cell communication induces random spikes of spontaneous calcium oscillations in multi-BV-2 microglial cells.
Cell-cell communication induces random spikes of spontaneous calcium oscillations in multi-BV-2 microglial cells.
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DOI：
10.1016/j.bbrc.2013.01.064
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发表时间：
2013-02
影响因子：
3.1
通讯作者：
Xian Wu;Leiting Pan;Y. Liu;Pengchong Jiang;Imshik Lee;I. Drevenšek‐Olenik;Xinzheng Zhang;Jingjun Xu
中科院分区：
文献类型：
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作者：
Xian Wu;Leiting Pan;Y. Liu;Pengchong Jiang;Imshik Lee;I. Drevenšek‐Olenik;Xinzheng Zhang;Jingjun Xu
作为中枢神经系统主动免疫防御的首要和主要形式，小胶质细胞通常表现出复杂的细胞内钙（Ca2+）活性，可以调节信号级联的下游组分。在本工作中，通过视频显微镜观察了多bv -2小胶质细胞胞质钙浓度（[Ca2+]c）的自发振荡。这些细胞表现出Ca2+振荡的随机峰值。对振荡时间依赖性的互相关分析表明，存在由细胞外信使介导的细胞间通讯。基于简单数学模型的数值模拟表明，这些通信可以在多细胞系统中诱导自发Ca2+振荡的随机峰值。对单个细胞不同区域随机峰的短时成像分析表明，自发的Ca2+振荡是由其他细胞产生的Ca2+波以及细胞内钙升高引起的。综上所述，我们的数据表明体外BV-2小胶质细胞之间存在细胞间通信，并进一步导致自发Ca2+振荡的随机峰值。
As the first and main form of active immune defense in the central nervous system, microglial cells usually exhibit complicated intracellular calcium (Ca2+) activity that can regulate the downstream components of signaling cascades. In the present work, spontaneous oscillations of the cytosolic calcium concentration ([Ca2+]c) in multi-BV-2 microglial cells were observed by video microscopy. These cells exhibited random spikes of Ca2+oscillations. Cross-correlation analysis of the temporal dependence of the oscillations indicated the existence of cell–cell communication mediated by extracellular messengers. Numerical simulations based on a simple mathematical model suggested that these communications could induce random spikes of spontaneous Ca2+oscillations in the multi-cell system. Short-time imaging analysis of random spikes in different regions of a single cell showed that spontaneous Ca2+oscillations resulted from Ca2+wave generated by other cells as well as from calcium elevation inside the cell. Taken together, our data demonstrate that cell–cell communication existed between the BV-2 microglial cells in vitro and further resulted in the random spikes of spontaneous Ca2+oscillations.