Seroprevalence of dengue IgG antibodies in symptomatic and asymptomatic individuals three years after an outbreak in Zhejiang Province, China
Seroprevalence of dengue IgG antibodies in symptomatic and asymptomatic individuals three years after an outbreak in Zhejiang Province, China
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DOI：
10.1186/s12879-018-3000-5
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发表时间：
2018-02-23
影响因子：
3.7
通讯作者：
Sun, Jimin
中科院分区：
文献类型：
--
作者：
Luo, Shuying;Cui, Weihong;Sun, Jimin
背景：交叉反应抗体增强人类登革热感染和抗体依赖增强(ADE)被认为是DHF/DSS的早期机制。然而，登革热抗体在体内的持续时间及其相关因素尚不清楚。方法：采集59名登革热症状患者和48名无症状患者的血样。研究参与者的人口统计信息(包括2009年的年龄、性别和居住地)也被收集起来。血清标本用Panbio登革热抗体间接酶联免疫吸附试验(ELISA)检测登革热特异性抗体。应用社会科学统计软件包对不同性别、年龄组、有症状或无症状感染的登革热血清抗体阳性率进行卡方检验和Logistic回归分析。结果：总体而言，70份(65.42%)血样的登革热抗体阳性率与按性别和不同年龄组的登革热抗体水平相近。经多因素Logistic回归分析，有登革热症状的人群血清抗体阳性率显著高于无症状人群(96.61%vs27.08%)，优势比(OR)为76.731。结论：多数个体在感染3年后即可检出登革热抗体。有登革热症状的人与无症状的人相比，登革热免疫球蛋白的感染率更高。
Background: Cross-reacting antibodies enhanced dengue infection in humans and antibody dependent enhancement (ADE) have been proposed as early mechanisms underlying DHF/DSS. However, the duration of dengue IgG antibodies in the body as well as factors associated with said duration remain unclear.Methods: Blood samples from 59 dengue symptomatic persons and 48 asymptomatic individuals were collected. Study participant demographic information (including age in 2009, gender, and place of residence) were also collected. Serum samples were tested for dengue specific IgG by Panbio dengue IgG indirect enzyme-linked immunosorbent assay (ELISA). Chi-square tests and logistic regression analysis of dengue IgG antibodies seroprevalence divided by gender, age groups, and symptomatic or asymptomatic infection were conducted using the Statistical Package for the Social Sciences.Results: Overall, 70 (65.42%) blood samples were seropositive for dengue IgG antibodies with similar seroprevalences found when dividing by gender and different age groups. However, seroprevalence of dengue IgG antibodies in samples from dengue symptomatic persons was significantly higher than that in samples from asymptomatic individuals (96.61% vs 27.08%) according to multivariable logistic regression analysis, the odds ratio (OR) of the factor was 76.731.Conclusions: Dengue IgG antibodies were detectable in samples from most individuals three years after infection. Dengue symptomatic persons had a higher dengue IgG prevalence compared to asymptomatic individuals.