Haloferax larsenii sp. nov., an extremely halophilic archaeon from a solar saltern.
Haloferax larsenii sp. nov., an extremely halophilic archaeon from a solar saltern.
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DOI：
10.1099/ijs.0.64573-0
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发表时间：
2007-04
影响因子：
2.8
通讯作者：
Xue-Wei Xu;Yue-hong Wu;Chun-sheng Wang;A. Oren;P. Zhou;Min Wu
中科院分区：
文献类型：
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作者：
Xue-Wei Xu;Yue-hong Wu;Chun-sheng Wang;A. Oren;P. Zhou;Min Wu
从浙江省某太阳盐湖分离到革兰氏阴性、好氧、嗜中性、极嗜盐古菌ZJ206(T)、ZJ203和ZJ204。在表型和16S rRNA基因序列上，菌株非常相似。比较16S rRNA基因分析显示，其序列相似性为96.4 ~ 97.4%。主要极性脂类是磷脂酰甘油的C(20)C（20）衍生物、磷脂酰甘油磷酸甲酯、二糖基甘油二醚和硫酸二糖基二醚。菌株ZJ206(T) DNA G+C含量为62.2 mol%。DNA-DNA杂交和生理生化测试的结果允许从近缘种分离的基因型和表型分化。因此，这些分离物应归类为一个新种，并建议将其命名为Haloferax larsenii sp. 11。型应变为ZJ206(T) (=CGMCC 1.5347（T)=JCM 13917(T)）。
Three strains of Gram-negative, aerobic, neutrophilic, extremely halophilic archaea, designated ZJ206(T), ZJ203 and ZJ204, were isolated from a solar saltern in Zhe-Jiang Province, China. Phenotypically and on the basis of 16S rRNA gene sequences, the strains were very similar. Comparative 16S rRNA gene analysis revealed 96.4-97.4 % sequence similarity to members of the genus Haloferax. The major polar lipids were C(20)C(20) derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, diglycosyl glycerol diether and sulfated diglycosyl diether. The DNA G+C content of strain ZJ206(T) was 62.2 mol%. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the isolates from closely related species. Therefore the isolates should be classified as members of a novel species, for which the name Haloferax larsenii sp. nov. is proposed. The type strain is ZJ206(T) (=CGMCC 1.5347(T)=JCM 13917(T)).