Vibrational and structural investigations on adipose tissues
Vibrational and structural investigations on adipose tissues
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DOI：
10.1080/14786430802585141
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发表时间：
2008-01-01
影响因子：
1.6
通讯作者：
Sbarbati, A.
中科院分区：
文献类型：
--
作者：
Giarola, M.;Guella, G.;Sbarbati, A.
在包括人类在内的哺乳动物中发现了两种类型的脂肪组织：白色脂肪组织（WAT）和棕色脂肪组织（BAT）。 WAT在脂质储存和身体隔热方面发挥着重要作用，而BAT是一种产热组织，通过氧化脂肪酸产生热量。这些不同脂肪组织的结构表征和光谱辨别都是当前感兴趣的问题，也考虑到可能的医学和生物技术应用。在这项工作中，通过红外和拉曼显微光谱对大鼠 BAT 和 WAT 进行了振动和结构研究。通过比较它们的振动光谱，一些特征峰已被确定为区分两种组织的有用标记。此外，还对WAT和BAT中甘油三酯成分的相对不饱和度进行了定量评估。这些结果证实了振动光谱作为生物和医学研究中可靠的微创工具。
Two types of adipose tissue are found in mammals, including humans: the white adipose tissue (WAT) and the brown adipose tissue (BAT). The WAT has a major role in lipid storage and body thermal insulation, while the BAT is a thermogenic tissue that produces heat by oxidizing fatty acids. Both structural characterization and spectroscopic discrimination of these different adipose tissues are matters of current interest, also in view of possible medical and bio-technological applications. In this work, vibrational and structural investigations on rat BAT and WAT have been carried out by means of infrared and Raman micro-spectroscopy. By comparing their vibrational spectra some characteristic peaks have been identified as useful markers to discriminate between the two tissues. Moreover, a quantitative evaluation of the relative degree of unsaturation of the triglyceride component in WAT and BAT has been derived. These results corroborate the vibrational spectroscopy as a reliable and minimally invasive tool in biological and medical research.