Roles of Toll-like receptors 2 and 6 in the inflammatory response to Mycoplasma gallisepticum infection in DF-1 cells and in chicken embryos
Roles of Toll-like receptors 2 and 6 in the inflammatory response to Mycoplasma gallisepticum infection in DF-1 cells and in chicken embryos
复制标题
Toll 样受体 2 和 6 在 DF-1 细胞和鸡胚胎鸡毒支原体感染炎症反应中的作用
DOI：
10.1016/j.dci.2016.01.008
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发表时间：
2016-06-01
影响因子：
2.9
通讯作者：
Peng, Xiuli
中科院分区：
文献类型：
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作者：
Tian, Wei;Zhao, Chengcheng;Peng, Xiuli
鸡毒支原体（Mycoplasma gallisepticum，MG）是引起鸡慢性呼吸道疾病的主要病原体，其感染的分子机制尚不清楚。本研究旨在探讨Toll样受体2（TLR 2）和TLR 6（TLR 6）在MG感染中的作用。我们发现TLR 2 -2和TLR 6在鸡胚成纤维细胞（DF-1细胞）中有差异表达，其中TLR 6高表达，而TLR 2 -2几乎不表达。MG感染后，TLR 6表达上调，随后是下游因子MyD 88、NF-κ B、IL 2、IL 6和TNF-α的上调。通过shRNA敲低TLR 6表达可消除MG诱导的炎症反应。更有趣的是，在TLR 6存在下，TLR 2 -2对MG感染DF-1细胞没有反应。然而，当TLR 6被shRNA敲低时，TLR 2在MG感染后上调，随后是促炎基因的上调。最后，我们检测了MG感染对鸡胚肺和气管组织中TLR 2 -2和TLR 6表达的影响。我们发现TLR 2 -2和TLR 6在MG感染后上调，随后上调下游NF-κ B介导的炎症反应。本研究首次报道了TLR 2 -2和TLR 6在MG感染的DF-1细胞和鸡胚中的不同作用。(C)2016爱思唯尔有限公司版权所有。
While Mycoplasma gallisepticum (MG) is a major pathogen that causes chronic respiratory diseases in chicken, the molecular mechanism of MG infection is not clear. In this study, we investigated the roles of Toll-like receptor 2 (TLR2) and 6 (TLR6) in MG infection. We found that TLR2 type 2 (TLR2-2) and TLR6 had differential expressions in chicken embryo fibroblasts (DF-1 cells), where TLR6 was highly expressed, but TLR2-2 was barely expressed. Upon MG infection, TLR6 expression was upregulated, followed by upregulation of downstream factors, MyD88, NF-kappa B, IL2, IL6, and TNF-alpha. Knockdown of TLR6 expression by shRNA abolished the MG-induced inflammatory responses. More interestingly, in the presence of TLR6, TLR2-2 didn't respond to MG infection in DF-1 cells. When TLR6 was knocked down by shRNA, however, TLR2 was upregulated upon MG infection, which was followed by upregulation of proinflammatory genes. Finally, we tested effects of the MG infection on expression of TLR2-2 and TLR6 in the lungs and trachea tissues of chicken embryos. We found both TLR2-2 and TLR6 were upregulated upon MG infection, followed by upregulation of the downstream NF-kappa B-mediated inflammatory responses. This study was the first to report the differential roles of TLR2-2 and TLR6 in MG-infected DF-1 cells and chicken embryos. (C) 2016 Elsevier Ltd. All rights reserved.