Developmental expression of inositol 1, 4, 5-trisphosphate receptor in the post-natal rat cochlea.
Developmental expression of inositol 1, 4, 5-trisphosphate receptor in the post-natal rat cochlea.
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出生后大鼠耳蜗中肌醇1,4,5-三磷酸受体的发育表达
DOI：
10.4081/ejh.2015.2486
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发表时间：
2015-04-21
期刊：
影响因子：
--
通讯作者：
Yang J
中科院分区：
文献类型：
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作者：
Liu WJ;Yang J
肌醇1，4，5-三磷酸受体（Inositol 1，4，5-trisphosphate receptor，IP 3R）是一种重要的听觉受体。然而，IP 3R在听觉发育期耳蜗中的表达尚不清楚。我们研究了IP 3R的表达在发育中的大鼠耳蜗使用免疫组织化学和实时逆转录聚合酶链反应（RT-PCR）。我们观察了它的存在，在发育中的大鼠耳蜗，和IP 3R蛋白表达的变化，从出生后早期到成年。出生时（出生后第0天，P0），IP 3R仅在Hensen细胞中表达。IP 3R免疫反应在P2时首先出现在Corti器的感觉毛细胞中。通过与耳蜗毛细胞的标记物肌球蛋白VIIA的双标记实验证实了这种定位。IP 3R和肌球蛋白VIIA从P2到出生后第二周的共定位表明IP 3R在发育中的内毛细胞和外毛细胞中的早期表达。从P8开始，螺旋韧带附近的Claudius细胞被IP 3R标记。IP 3R在出生后早期的大鼠血管纹中的瞬时表达从P4到P8。螺旋神经节神经元也表现出较弱的IP 3R荧光信号在出生后的发展。RT-PCR结果表明，所有三种IP 3R异构体（IP 3R 1，IP 3R 2和IP 3R 3）存在于大鼠耳蜗的四个不同的发育阶段，从P0到P28。目前的免疫组织化学证据表明，在出生后的发展过程中的大鼠耳蜗IP 3R的表达的变化和维护表明，可能参与的IP 3R介导的钙信号在耳蜗发育。
Inositol 1, 4, 5-trisphosphate receptor (IP3R) has been established to be essential for hearing. However, the expression of IP3R in the cochlea in the period of auditory development remains unknown. We investigated the expression of IP3R in the developing rat cochlea using immunohistochemistry and real-time reverse transcription polymerase chain reaction (RT-PCR). We observed its presence in the developing rat cochlea, and changes in IP3R protein expressions from the early post-natal period to adult. At birth (postnatal day 0, P0), IP3R expression was only found in Hensen’s cell. IP3R immunoreactivity first appeared in the sensory hair cells in the organ of Corti at P2. This localization was confirmed by means of double-labeling experiments with Myosin VIIA, a marker for cochlear hair cells. Colocalization of IP3R and Myosin VIIA from P2 to the second post-natal week suggested early expression of IP3R in developing inner and outer hair cells. Claudius’ cells near the spiral ligament were labelled for IP3R from P8 onwards. Transient IP3R expression was observed in the stria vascularis in early post-natal rat from P4 to P8. Spiral ganglion neurons also exhibited weaker IP3R fluorescence signals during post-natal development. The results of RT-PCR demonstrated that all three IP3R isoforms (IP3R1, IP3R2, and IP3R3) were present in rat cochlea during four different developmental stages of cochlea, from P0 to P28. Present immunohistochemical evidence for both change and maintenance of expression of IP3R during post-natal development of the rat cochlea indicated the possible involvement of IP3R-mediated calcium signaling in cochlear development.