Neuronal expression of mint1 and mint2, novel multimodular proteins, in adult murine brain
Neuronal expression of mint1 and mint2, novel multimodular proteins, in adult murine brain
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DOI：
10.1016/s0169-328x(01)00126-7
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发表时间：
2001-08-15
期刊：
影响因子：
--
通讯作者：
Matsuyama, T
中科院分区：
文献类型：
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作者：
Nakajima, Y;Okamoto, M;Matsuyama, T
薄荷糖是功能膜运输和组织的多模块适配器蛋白。Mint1和Mint2是神经元特异性的。我们在小鼠脑中定位了这些异构体。通过原位杂交，编码mint 1或mint 2的mRNA在整个大脑的神经元中表达。Mint 1 mRNA在边缘系统包括扣带回皮质、海马、丘脑前核、内侧缰核和乳头体中表达最高，Mint 2 mRNA在大脑皮质、内嗅皮质和海马中表达丰富，但在边缘系统其他结构中表达不明显。mint 1和mint 2 mRNA分布于海马锥体神经元，而mint 2 mRNA在CA3区尤为丰富。Mint 1 mRNA在黑质腹侧部表达丰富，而Mint 2 mRNA在黑质腹侧部表达不丰富。免疫组织化学可视化薄荷蛋白轴突终末和神经元胞体，一般以下mRNA的分布。在海马，薄荷1是丰富的内嗅突起和苔藓纤维的齿状回，而薄荷2是丰富的连合纤维从对侧海马和CAI。Mint1强烈染色的含儿茶酚胺神经元，如黑质部，腹侧被盖区，蓝斑。Mint2蛋白在这些区域普遍存在。Mint1和Mint2在脑干和小脑中的分布也不同。中枢神经系统神经元主要表达mint 1或mint 2。薄荷可能参与突触囊泡向活动区的运输，也参与某些膜蛋白向突触后密度的运输。Mint1和Mint2可能在区域上或根据神经元功能特征划分角色。(C)2001 Elsevier Science BY保留所有权利。
Mints are multimodular adapter proteins in functioning membrane transport and organization. Mint1 and mint2 are neuron-specific. We localized these isoforms in mouse brain. By in situ hybridization, mRNA encoding mint1 or mint2 was expressed in neurons throughout the brain. Mint1 mRNA expression was greatest in the limbic system including cingulate cortex, hippocampus, anterior thalamic nuclei, medial habenular nucleus, and mammillary body, Mint2 mRNA was rich in cerebral cortex, entorhinal cortex, and hippocampus, but less prominent in other limbic structures. Mint1 mRNA and mint2 mRNA were distributed among hippocampal pyramidal neurons, while mint2 mRNA was especially abundant in CA3. Mint1, but not Mint2 mRNA was abundant in the substantia nigra pars compacta. Immunohistochemistry visualized mint proteins in axon terminals and neuronal somata, generally following mRNA distribution. In the hippocampus, mint1 was rich in the entorhinal projections and mossy fibers of the dentate gyrus, while mint2 was rich in commisural fibers from the contralateral hippocampus and in CAI. Mint1 intensely stained catecholamine-containing neurons such as the substantia nigra pars compacta, ventral tegmental area, and locus ceruleus. Mint2 protein was ubiquitous in these regions. Mint1 and mint2 distribution also differed elsewhere in the brainstem and in the cerebellum. Central nervous system neurons, then, predominantly express either mint1 or mint2. Mints may be involved in synaptic vesicle transport toward the active zone, also participating in transport of certain membrane proteins toward the postsynaptic density. Mint1 and mint2 may divide roles either regionally or depending on neuronal functional characteristics. (C) 2001 Elsevier Science BY All rights reserved.