Antibiotic susceptibility profile of bacteria isolated from natural sources of water from rural areas of East sikkim.
Antibiotic susceptibility profile of bacteria isolated from natural sources of water from rural areas of East sikkim.
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DOI：
10.4103/0970-0218.137152
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发表时间：
2014-07-01
期刊：
影响因子：
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通讯作者：
Tsering, Dechen C
中科院分区：
文献类型：
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作者：
Poonia, Shubra;Singh, T Shantikumar;Tsering, Dechen C
背景技术背景：水、食品和环境被耐药细菌污染是一个严重的公共卫生问题。目的：研究东锡金自然水源的细菌污染，并确定细菌分离株的抗菌特性。材料和方法：采用薄膜过滤法对同一来源的225份样品进行细菌学分析，其中冬、夏、季风季节各75份。结果：共分离出埃希氏菌属、克雷伯氏菌属、变形杆菌属、沙门氏菌属、志贺氏菌属、肠杆菌属、柠檬酸杆菌属、摩根菌属、假单胞菌属、不动杆菌属、黄杆菌属、沙雷氏菌属等19种细菌，并进行了药敏试验。一般来说，除沙门氏菌和志贺氏菌属外，大多数细菌分离株对常用抗生素如氨苄青霉素具有耐药性（57.5%），甲氧苄啶/磺胺甲氧唑（39.1%），阿莫西林/克拉维酸（37.4%），头孢克肟（34.5%），四环素（29.1%），头孢他啶（26.3%），氧氟沙星（25.9%），阿米卡星（8.7%）和庆大霉素结论：锡金东部天然水源严重污染，包括肠道病原菌。从这些来源饮用未经处理的水可能会对消费者的健康构成威胁。
BACKGROUND: Contamination of water, food, and environment with antibiotic-resistant bacteria poses a serious public health issue.OBJECTIVE: The objective was to study the bacterial pollution of the natural sources of water in east Sikkim and to determine the antimicrobial profile of the bacterial isolates.MATERIALS AND METHODS: A total of 225 samples, 75 each during winter, summer, and monsoon season were collected from the same source in every season for bacteriological analysis by membrane filtration method. Antibiotic susceptibility test was performed using standard disc diffusion method.RESULTS: A total of 19 bacterial species of the genera Escherichia, Klebsiella, Proteus, Salmonella, Shigella, Enterobacter, Citrobacter, Morganella, Pseudomonas, Acinetobacter, Flavobacterium, and Serratia were isolated and their antimicrobial sensitivity tested. Generally, most bacterial isolates except Salmonella and Shigella species were found resistant to commonly used antibiotics such as ampicillin (57.5%), trimethoprim/sulfamethoxaole (39.1%), amoxicillin/clavulanic acid (37.4%), cefixime (34.5%), tetracycline (29.1%), ceftazidime (26.3%), ofloxacin (25.9%), amikacin (8.7%), and gentamicin (2.7%) but sensitive to imipenem and piperacillin/tazobactam.CONCLUSION: Natural sources of water in east Sikkim are grossly contaminated with bacteria including enteropathogens. The consumption of untreated water from these sources might pose health risk to consumers.