Biodegradable alginate microspheres as a delivery system for naked DNA.

Biodegradable alginate microspheres as a delivery system for naked DNA.
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DOI：
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发表时间：
1999
期刊：
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
影响因子：
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通讯作者：
N. Aggarwal;H. Hogenesch;P. Guo;A. North;M. Suckow;S. Mittal
N. Aggarwal;H. Hogenesch;P. Guo;A. North;M. Suckow;S. Mittal
中科院分区：
其他
文献类型：
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作者：
N. Aggarwal;H. Hogenesch;P. Guo;A. North;M. Suckow;S. Mittal

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Sodium alginate is a naturally occurring polysaccharide that can easily be polymerized into a solid matrix to form microspheres. These biodegradable microspheres were used to encapsulate plasmid DNA containing the bacterial beta-galactosidase (LacZ) gene under the control of either the cytomegalovirus (CMV) immediate-early promoter or the Rous sarcoma virus (RSV) early promoter. Mice inoculated orally with microspheres containing plasmid DNA expressed LacZ in the intestine, spleen and liver. Inoculation of mice with microspheres containing both the plasmid DNA and bovine adenovirus type 3 (BAd3) resulted in a significant increase in LacZ expression compared to those inoculated with microspheres containing only the plasmid DNA. Our results suggest that adenoviruses are capable of augumenting transgene expression by plasmid DNA both in vitro and in vivo.