B lymphocytes play a limited role in clearance of Campylobacter jejuni from the chicken intestinal tract.
B lymphocytes play a limited role in clearance of Campylobacter jejuni from the chicken intestinal tract.
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DOI：
10.1038/srep45090
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发表时间：
2017-03-23
影响因子：
4.6
通讯作者：
Wigley P
中科院分区：
文献类型：
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作者：
Lacharme-Lora L;Chaloner G;Gilroy R;Humphrey S;Gibbs K;Jopson S;Wright E;Reid W;Ketley J;Humphrey T;Williams N;Rushton S;Wigley P
空肠弯曲菌是食源性细菌性胃肠炎的主要原因，受污染的禽肉是其主要来源。控制C.空肠弯曲杆菌是家禽业的优先考虑对象，但是没有疫苗可用，并且由于对空肠弯曲杆菌的免疫生物学缺乏了解而阻碍了疫苗的开发。空肠感染在这里，我们展示了B淋巴细胞在C反应中的功能作用。通过耗尽B淋巴细胞群（法氏囊切除术），然后进行攻击，在鸡空肠中进行。B淋巴细胞耗竭对盲肠中的细菌数量几乎没有影响，盲肠是细菌定植的主要部位，而C.空肠持续到超过商业屠宰年龄，但减少从小肠的清除。在长期实验中，我们发现抗体导致C。感染后9周盲肠中的Jeuni数。虽然我们没有检查任何保护作用，再挑战，它说明了在一个年轻的，免疫幼稚的宿主生产疫苗的困难。我们认为这是首次对C.结果表明，抗体对鸡空肠弯曲菌的清除效果不明显。在鸡的生产寿命内，空肠从盲肠中清除，尽管参与小肠的清除和盲肠的长期清除。
Campylobacter jejuni is the leading cause of foodborne bacterial gastroenteritis with contaminated poultry meat its main source. Control of C. jejuni is a priority for the poultry industry but no vaccines are available and their development hampered by poor understanding of the immunobiology of C. jejuni infection. Here we show the functional role of B lymphocytes in response to C. jejuni in the chicken through depletion of the B lymphocyte population (bursectomy) followed by challenge. B lymphocyte depletion has little effect on bacterial numbers in the ceca, the main site of colonisation, where C. jejuni persist to beyond commercial slaughter age, but reduces clearance from the small intestine. In longer-term experiments we show antibody leads to reduction in C. jeuni numbers in the ceca by nine weeks post infection. Whilst we did not examine any protective role to re-challenge, it illustrates the difficulty in producing a vaccine in a young, immunologically naïve host. We believe this is first study of functional immunity to C. jejuni in chicken and shows antibody is ineffective in clearing C. jejuni from the ceca within the production lifetime of chickens, although is involved in clearance from the small intestine and longer-term clearance from the ceca.