Evidence that glycocalicin circulates in normal plasma.
Evidence that glycocalicin circulates in normal plasma.
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糖运载蛋白在正常血浆中循环的证据。
DOI：
10.1172/jci111273
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Scudder,LE
中科院分区：
文献类型：
--
作者：
Coller,BS;Kalomiris,E;Steinberg,M;Scudder,LE
通过使用一种异源抗血清GPIb/glycocalicin和一种放射性标记的单克隆抗体GPIb/glycocalicin的组合，我们能够开发一种敏感和特异性的放射免疫电泳分析，可以区分少量的glycocalicin从GPIb。正常血浆中发现含有glycocalicin，即使在用蛋白酶抑制剂处理并广泛离心以除去血小板和血小板碎片的样品中也是如此。从采用凝胶色谱和亲和色谱的研究中确认血浆抗原具有与糖帽蛋白相似或相同的相对分子量。开发了免疫放射测定法来定量血浆糖帽蛋白，发现正常血浆含有约1-3微克/ml。再生障碍性贫血导致的严重血小板减少症患者的血浆中的glycocalicin水平低于正常水平的12.5%，而两名血小板减少症患者由于血小板破坏增加（特发性血小板减少性紫癜和溶血性尿毒症综合征）的疾病有正常水平。因此，在体内似乎存在血小板GPIb的持续catenopathy，我们推测，血浆glycocalicin水平反映了一个复杂的功能因素，包括血小板计数，血小板周转，和血小板破坏的网站。图片
By using a combination of a heterologous antiserum to GPIb/glycocalicin and a radiolabeled monoclonal antibody to GPIb/glycocalicin, we were able to develop a sensitive and specific radioimmunoelectrophoretic assay that can distinguish small amounts of glycocalicin from GPIb. Normal plasmas were found to contain glycocalicin, even in samples treated with protease inhibitors and centrifuged extensively to remove platelets and platelet fragments. Confirmation that the plasma antigen had a relative molecular weight similar or identical to glycocalicin was obtained from studies employing gel chromatography and affinity chromatography. An immunoradiometric assay was developed to quantify plasma glycocalicin, and normal plasma was found to contain approximately 1-3 micrograms/ml. The plasma of a patient with severe thrombocytopenia due to aplastic anemia had less than 12.5% of the normal level of glycocalicin, whereas two patients with thrombocytopenia due to diseases of increased platelet destruction (idiopathic thrombocytopenic purpura and hemolytic-uremic syndrome) had normal levels. Thus, there appears to be ongoing catabolism of platelet GPIb in vivo, and we postulate that the plasma level of glycocalicin reflects a complex function of factors, including platelet count, platelet turnover, and the site of platelet destruction.Images