Norethindrone alters mating behaviors, ovary histology, hormone production and transcriptional expression of steroidogenic genes in zebrafish (Danio rerio)
Norethindrone alters mating behaviors, ovary histology, hormone production and transcriptional expression of steroidogenic genes in zebrafish (Danio rerio)
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炔诺酮改变斑马鱼（斑马鱼）的交配行为、卵巢组织学、激素产生和类固醇基因的转录表达
DOI：
10.1016/j.ecoenv.2020.110496
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发表时间：
2020
影响因子：
6.8
通讯作者：
Xie Lingtian
中科院分区：
文献类型：
--
作者：
Hou Liping;Chen Sh;uo;Shi Wenjun;Chen Hongxing;Liang Yanqiu;Wang Xiaolan;Tan Jiefeng;Wang Yifan;Deng Xikai;Zhan Manjun;Long Jianzhao;Cai Guowei;Luo Shaowen;Zhang Cuiping;Liu Juan;Leung Jonathan Y. S.;Xie Lingtian
孕激素（即孕酮的合成形式）对水生生物的影响越来越引起人们的关注，因为它们广泛存在于水生环境中，并对鱼类的内分泌系统产生潜在影响。在这项研究中，通过将雌性斑马鱼暴露于0、3.1、36.2和398.6 ng L− 1的炔诺酮（NET，一种雌激素）60天，评估了炔诺酮对生殖行为、性激素产生和转录表达的影响。结果表明，NET在36.2和398.6 ng L− 1时损害了雄性表现出的雌性交配行为，并增加了暴露于398.6 ng L−1 NET的卵巢中闭锁卵泡细胞的频率。性激素方面，斑马鱼血浆睾酮浓度升高，雌二醇浓度降低。在接触398.6 ng L−1 NET的鱼脑中检测到基因（Npr、Mpra、Mprβ、FSHβ、Lβ、Tshb、NisandDio2）上调。NET在398.6 ng L−1时，通常会抑制斑马鱼卵巢中基因（Esr 1、Vtg 1、Ar、Cyp19 a、Cyp11和Ptgs 2）的转录水平。此外，NET在36.2和398.6 ng L−1时减少了肝脏中基因（Vtg1，Esr1，ArandPgr）的转录。我们的研究结果表明，NET不仅可以通过破坏它们的生殖器官，而且还可以通过改变负责产生性激素的基因的表达来改变它们的交配行为，从而潜在地减少鱼类种群的数量。
The impact of progestins (i.e. synthetic forms of progesterone) on aquatic organisms has drawn increasing attention due to their widespread occurrence in the aquatic environments and potential effects on the endocrine system of fish. In this study, the effects of norethindrone (NET, a progestin) on the reproductive behavior, sex hormone production and transcriptional expressions were evaluated by exposing female zebrafish to NET at 0, 3.1, 36.2 and 398.6 ng L−1for 60 days. Results showed that NET impaired the mating behaviors of female at 36.2 and 398.6 ng L−1exhibited by males and increased the frequency of atretic follicular cells in the ovary exposed to NET at 398.6 ng L−1. As for sex hormones, plasma testosterone concentration in zebrafish increased, while estradiol concentration decreased. Up-regulation of genes (Npr, Mpra, Mprβ, Fshβ, Lβ, Tshb, NisandDio2) was detected in the brain of fish exposed to NET at 398.6 ng L−1. The transcriptional levels of genes (Esr1, Vtg1, Ar, Cyp19a, Cyp11bandPtgs2)were generally inhibited in the ovary of zebrafish by NET at 398.6 ng L−1. Moreover, the transcripts of genes (Vtg1, Esr1, ArandPgr) in the liver were reduced by NET at 36.2 and 398.6 ng L−1. Our findings suggest that NET can potentially diminish the of fish populations not only by damaging their reproductive organs, but also by altering their mating behavior through the changes in the expressions of genes responsible for the production of sex hormones.