A fluorescent aptasensor based on copper nanoclusters for optical detection of CD44 exon v10, an important isoform in metastatic breast cancer
A fluorescent aptasensor based on copper nanoclusters for optical detection of CD44 exon v10, an important isoform in metastatic breast cancer
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DOI：
10.1039/d1ay01087e
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发表时间：
2021-07-22
影响因子：
3.1
通讯作者：
Hosseinkhani, Saman
中科院分区：
文献类型：
--
作者：
Bahmani, Amin;Shokri, Ehsan;Hosseinkhani, Saman
最近的研究表明，乳腺癌细胞表达多种CD44亚型。CD44是一种由单个20外显子基因编码的完整跨膜蛋白。CD44基因外显子V10在促进肿瘤转移中起着重要作用，因此对该亚型的敏感检测有助于转移性乳腺癌的早期诊断和治疗。本研究旨在利用V10特异性适配子构建基于荧光金属纳米团簇的光学适配子传感器。为此，用不同的CD44 V10 DNA适配子作为分子模板制备了银、金和铜的纳米团簇。紫外-可见光谱、透射电子显微镜和荧光光谱仪的结果证实了合成的适体模板纳米簇(APT-NCS)的准确性和质量。最后，我们比较了所制备的APT-NCS对不同培养细胞系的响应性能。结果表明，M-Apt4-CuNC的光响应效率更高，并且与CD44V10-细胞的浓度有很好的相关性。该适配子传感器的检测下限为每毫升40+/-5个细胞。
Recent studies suggest that breast cancer cells express various CD44 isoforms. CD44 is an integral transmembrane protein encoded by a single 20-exon gene. Exon v10 of CD44 plays a critical role in promoting cancer metastasis, so sensitive detection of this isoform helps in early diagnosis of metastatic breast cancer and facilitates the treatment process. This study aimed to use v10-specific aptamers to set up an optical aptasensor based on fluorescent metal nanoclusters. For this purpose, nanoclusters of silver, gold, and copper were prepared by different CD44 v10 DNA aptamers as molecular templates. UV-vis, TEM, and fluorescence spectrometer results confirmed the accuracy and quality of the synthesized aptamer-templated nanoclusters (Apt-NCs). Finally, we compared the performance of the as-prepared Apt-NCs in response to different cultured cell lines. According to the results, the optical response of M-Apt4-CuNCs was more efficient and correlated well with the concentrations of CD44 v10-enriched cells. The detection limit of the aptasensor was 40 +/- 5 cells per mL.