Intestinal smooth muscle cell maintenance by basic fibroblast growth factor
Intestinal smooth muscle cell maintenance by basic fibroblast growth factor
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DOI：
10.1089/ten.tea.2007.0232
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发表时间：
2008-08-01
影响因子：
4.1
通讯作者：
Dunn, James C. Y.
中科院分区：
文献类型：
--
作者：
Lee, Min;Wu, Benjamin M.;Dunn, James C. Y.
肠组织工程是治疗短肠综合征的一种有效方法。促进平滑肌细胞增殖和血管生成的组织工程支架对于功能性平滑肌的再生和运动是必不可少的。由于碱性成纤维细胞生长因子（bFGF）可以刺激平滑肌增殖和血管生成，bFGF的递送被用来刺激原代肠平滑肌细胞的增殖和存活。两种方法的局部bFGF的交付进行了检查：将bFGF的胶原蛋白涂层和封装成聚（D，L-乳酸-羟基乙酸）微球的bFGF。将细胞接种的支架植入网膜中，并在4、14和28天后取回。在所有植入物中，接种的细胞从第4天增殖到第14天;然而，在第28天，与没有bFGF或在胶原中含有1 μ g bFGF的支架相比，当10 μ g bFGF掺入支架的胶原涂层中时，观察到植入细胞和血管的密度显著更高。1 μ g bFGF的微球包封产生与10 μ g bFGF混合在胶原中类似的效果，并且比通过胶原掺入递送1 μ g bFGF更有效。大多数植入的细胞也表达α-平滑肌肌动蛋白。用微球包裹bFGF并接种平滑肌细胞的支架可能是肠平滑肌再生的有用平台。
Intestinal tissue engineering is a potential therapy for patients with short bowel syndrome. Tissue engineering scaffolds that promote smooth muscle cell proliferation and angiogenesis are essential toward the regeneration of functional smooth muscles for peristalsis and motility. Since basic fibroblast growth factor (bFGF) can stimulate smooth muscle proliferation and angiogenesis, the delivery of bFGF was employed to stimulate proliferation and survival of primary intestinal smooth muscle cells. Two methods of local bFGF delivery were examined: the incorporation of bFGF into the collagen coating and the encapsulation of bFGF into poly(D,L-lactic-co-glycolic acid) microspheres. Cell-seeded scaffolds were implanted into the omentum and were retrieved after 4, 14, and 28 days. The seeded cells proliferated from day 4 to day 14 in all implants; however, at 28 days, significantly higher density of implanted cells and blood vessels was observed, when 10 mu g of bFGF was incorporated into the collagen coating of scaffolds as compared to scaffolds with either no bFGF or 1 mu g of bFGF in collagen. Microsphere encapsulation of 1 mu g of bFGF produced similar effects as 10 mu g of bFGF mixed in collagen and was more effective than the delivery of 1 mu g of bFGF by collagen incorporation. The majority of the implanted cells also expressed alpha-smooth muscle actin. Scaffolds coated with microsphere-encapsulated bFGF and seeded with smooth muscle cells may be a useful platform for the regeneration of the intestinal smooth muscle.