The hemolymph melanization response is related to defence against the AcMNPV infection in Bombyx mori
The hemolymph melanization response is related to defence against the AcMNPV infection in Bombyx mori
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家蚕血淋巴黑化反应与防御 AcMNPV 感染有关
DOI：
10.1002/arch.21764
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发表时间：
2021-07-16
影响因子：
2.2
通讯作者：
Li, Muwang
中科院分区：
文献类型：
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作者：
Li, Tao;Wang, Xueyang;Li, Muwang
黑素化是由酚氧化酶原（proPO）激活级联介导的，并且在节肢动物免疫系统中起重要作用。先前，我们发现p50株的血淋巴在感染苜蓿银纹夜蛾多核型多角体病毒（AcMNPV）后不进行黑化。然而，这一机制尚不清楚。本研究通过对家蚕AcMNPV敏感品系和抗性品系接种AcMNPV后血淋巴黑化抑制的分析，探讨了AcMNPV抑制血淋巴黑化的机理。结果表明，在接种后期（72 ~ 120 h），抗性品系C108的血淋巴黑化程度高于敏感品系p50。感染后期（72 ~ 120 hpi）PO活性显著降低，P50中BmPPO 1和BmPPO 2表达下调。而在抗性菌株C108中，PO活性升高，而BmPPO 1和BmPPO 2的表达量没有明显变化。BmPPAE基因的表达在病毒感染期间在两个菌株中上调。此外，血淋巴黑化可减弱病毒的体外活性。这些结果提示家蚕血淋巴黑化反应与防御AcMNPV感染有关。
Melanization is mediated by the prophenoloxidase (proPO) activation cascade and plays an important role in the arthropods immune system. Previously, we found that the hemolymph of the p50 strain does not perform melanization after infection with Autographa californica multiple nucleopolyhedrovirus (AcMNPV). However, this mechanism is still unclear. In this study, the underlying mechanism of the inhibition of hemolymph melanization was investigated by analysing the AcMNPV-susceptible or -resistant silkworm strains after inoculation with AcMNPV. The results showed that the level of hemolymph melanization was higher in resistant strain C108 than in susceptible strain p50 at the late stage (72 to 120 h postinoculation). The PO activity decreased significantly at the late stage of infection (72 to 120 hpi), and the expression of BmPPO1 and BmPPO2 was downregulated in p50. However, the PO activity increased in the resistant strain C108, while the expression level of BmPPO1 and BmPPO2 displayed no significant changes. The expression of the BmPPAE gene was upregulated in two strains during viral infection. In addition, the hemolymph melanization can weaken the viral activity in vitro. Our results suggested that the silkworm hemolymph melanization response is related to defence against the AcMNPV infection.