An NMR-based metabonomic investigation on effects of milk and meat protein diets given to 8-year-old boys
An NMR-based metabonomic investigation on effects of milk and meat protein diets given to 8-year-old boys
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DOI：
10.1017/s0007114507450322
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发表时间：
2007-04-01
影响因子：
3.6
通讯作者：
Michaelsen, Kim F.
中科院分区：
文献类型：
--
作者：
Bertram, Hanne Christine;Hoppe, Camilla;Michaelsen, Kim F.
这项研究的目的是调查基于核磁共振的代谢组学方法应用于生物体液的能力，以探索和识别青春期前儿童短期高摄入牛奶蛋白或肉类蛋白的总体外源性和内源性生化影响，目的是比较相对差异，而不是绝对量化。共有24名8岁男孩被要求每天摄入53g蛋白质作为牛奶(N 12)或肉类(N 12)。在基线和7d后，收集尿样和血清样本，并使用800 MHz光谱仪获取高分辨率(1)核磁共振氢谱。牛奶日粮减少了马尿酸的排泄，而肉类日粮增加了尿肌酸、组氨酸和尿素的排泄。对血清的核磁共振测量显示，血脂谱有轻微的变化，这最可能归因于摄入牛奶后血液中SCFA含量的增加。肉食对血清代谢谱无明显影响。这项研究首次证明了基于质子核磁共振的代谢组学能够识别不同动物蛋白消费的整体生化影响。由于短期饮食干预，尿代谢物谱比血清代谢物谱更容易受到干扰。牛奶饮食诱导的马尿酸排泄量的减少表明肠道微生物区系的改变，这可能为进一步研究牛奶中的生物活性成分的作用提供有用的信息。
The objective of the study was to investigate the ability of an NMR-based metabonomic approach, applied to biofluids, to explore and identify overall exogenous and endogenous biochemical effects of a short-time high intake of milk protein or meat protein given to prepubertal children, the aim being to compare relative differences and not an absolute quantification. A total of twenty-four 8-year-old boys were asked to take 53 g protein as milk (n 12) or meat daily (n 12). At baseline and after 7 d, urine and serum samples were collected and high-resolution (1)H NMR spectra were acquired on these using a 800 MHz spectrometer. The milk diet reduced the urinary excretion of hippurate, while the meat diet increased the urinary excretion of creatine, histidine and urea. The NMR measurements on serum revealed minor changes in the lipid profile, which most probably should be ascribed to an increase in the content of SCFA in the blood after consumption of the milk diet. The meat diet had no effect on the metabolic profile of serum. The study for the first time demonstrates the capability of proton NMR-based metabonomics to identify the overall biochemical effects of consumption of different animal proteins. The urine metabolite profile is more susceptible to perturbations as a result of short diet interventions than the serum metabolite profile. The milk diet-induced reduction in urinary excretion of hippurate suggests alterations in gut microflora, which may be useful information for further studies elucidating the effects of bioactive components in milk.