Bioactive silicate materials stimulate angiogenesis in fibroblast and endothelial cell co-culture system through paracrine effect
Bioactive silicate materials stimulate angiogenesis in fibroblast and endothelial cell co-culture system through paracrine effect
复制标题
生物活性硅酸盐材料通过旁分泌作用刺激成纤维细胞和内皮细胞共培养系统中的血管生成
DOI：
10.1016/j.actbio.2013.02.014
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发表时间：
2013-06-01
影响因子：
9.7
通讯作者：
Chang, J.
中科院分区：
文献类型：
--
作者：
Li, H.;Chang, J.
血管生成是组织工程中的关键，生物陶瓷诱导的血管生成已有报道。然而，其他类型的细胞如成纤维细胞在这种生物陶瓷诱导的血管生成过程中的作用尚未报道，并且更接近于组织再生的体内情况。在这项研究中，硅酸盐生物陶瓷诱导的血管生成在成纤维细胞存在的旁分泌作用，通过调查的影响，硅酸盐（CS），最简单的硅酸盐生物活性陶瓷之一，对血管生成的人皮肤成纤维细胞（HDF）和人脐静脉内皮细胞（HUVEC）的共培养物中的确认。结果显示，CS提取物刺激共培养HDF表达血管内皮生长因子（VEGF），并随后增强共培养HUVEC（co-HUVEC）上VEGF受体2的表达。然后增加co-HUVEC中的内皮型一氧化氮合酶和一氧化氮的产生以最终启动促血管生成。在此过程中，血管内皮钙粘蛋白的表达从co-HUVEC上调，和钙粘蛋白的蛋白集中在细胞连接，以促进管形成。已证实硅离子在硅酸盐生物陶瓷诱导血管生成过程中发挥重要作用，并提出了有效的硅离子浓度（0.7-1.8 μ g ml（-1））。(C)2013 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Angiogenesis is critical in tissue engineering, and bioceramic-induced angiogenesis has been reported. However, the role of other types of cells such as fibroblasts in this bioceramic-induced angiogenesis process has not been reported, and is closer to the in vivo situation of tissue regeneration. In this study, the paracrine effect of silicate bioceramic-induced angiogenesis in the presence of fibroblasts was confirmed by investigating the effects of calcium silicate (CS), one of the simplest silicate bioactive ceramics, on angiogenesis in co-cultures of human dermal fibroblasts (HDF) and human umbilical vein endothelial cells (HUVEC). Results showed that CS extracts stimulated the expression of vascular endothelial growth factor (VEGF) from co-cultured HDF and subsequently enhanced the expression of VEGF receptor 2 on co-cultured HUVEC (co-HUVEC). The endothelial nitric oxide synthase and nitric oxide production in co-HUVEC was then increased to finally initiate the proangiogenesis. During this process, the expression of vascular endothelial cadherin from co-HUVEC was up-regulated, and cadherin proteins were concentrated at the cell junctions to facilitate tube formation. Silicon ions are confirmed to play an important role during silicate bioceramic-inducing angiogenesis, and effective silicon ion concentrations (0.7-1.8 mu g ml(-1)) are proposed. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.