Nonstructural protein Pns12 of rice dwarf virus is a principal regulator for viral replication and infection in its insect vector
Nonstructural protein Pns12 of rice dwarf virus is a principal regulator for viral replication and infection in its insect vector
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水稻矮缩病毒的非结构蛋白 Pns12 是其昆虫载体中病毒复制和感染的主要调节因子
DOI：
10.1016/j.virusres.2015.07.012
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发表时间：
2015-12-02
期刊：
影响因子：
5
通讯作者：
Wei, Taiyun
中科院分区：
文献类型：
--
作者：
Chen, Qian;Chen, Hongyan;Wei, Taiyun
植物呼肠孤病毒被认为在称为病毒质的细胞质结构内复制和组装。水稻矮缩病毒（RDV）（一种植物呼肠孤病毒）在其叶蝉载体细胞中感染期间病毒质形成的分子机制仍知之甚少。病毒非结构蛋白Pns12在没有病毒感染的情况下形成病毒浆样包涵体，表明病毒浆基质基本上由Pns12组成。在这里，我们证明了核心衣壳蛋白 P3 和非结构蛋白 Pns11 通过与 Pns12 直接相互作用而被招募到病毒质中，而非结构蛋白 Pns6 通过与 Pns11 相互作用而被招募。将 Pns12 基因中的 dsRNA 引入培养的昆虫载体细胞或完整昆虫中，会强烈抑制这种病毒质的形成，从而阻止病毒在叶蝉体内和体外的有效传播。因此，RDV 的非结构蛋白 Pnsl 2 是其昆虫载体中病毒复制和感染的主要调节因子。 (C) 2015 Elsevier B.V. 保留所有权利。
Plant reoviruses are thought to replicate and assemble within cytoplasmic structures called viroplasms. The molecular mechanisms underling the formation of the viroplasm during infection of rice dwarf virus (RDV), a plant reovirus, in its leafhopper vector cells remain poorly understood. Viral nonstructural protein Pns12 forms viroplasm-like inclusions in the absence of viral infection, suggesting that the viroplasm matrix is basically composed of Pns12. Here, we demonstrated that core capsid protein P3 and nonstructural protein Pns11 were recruited in the viroplasm by direct interaction with Pns12, whereas nonstructural protein Pns6 was recruited through interaction with Pns11. The introduction of dsRNA from Pns12 gene into cultured insect vector cells or intact insect strongly inhibited such viroplasm formation, preventing efficient viral spread in the leafhopper in vitro and in vivo. Thus, nonstructural protein Pnsl 2 of RDV is a principal regulator for viral replication and infection in its insect vector. (C) 2015 Elsevier B.V. All rights reserved.