Molecular Tagging of a Major Quantitative Trait Locus for Broad-Spectrum Resistance to Verticillium Wilt in Upland Cotton Cultivar Prema
Molecular Tagging of a Major Quantitative Trait Locus for Broad-Spectrum Resistance to Verticillium Wilt in Upland Cotton Cultivar Prema
复制标题
陆地棉品种 Prema 广谱抗黄萎病的主要数量性状基因座的分子标记
DOI：
10.2135/cropsci2012.12.0694
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发表时间：
2013-11-01
期刊：
影响因子：
2.3
通讯作者：
Zhang, Tianzhen
中科院分区：
文献类型：
--
作者：
Ning Zhiyuan;Zhao, Rui;Zhang, Tianzhen
黄萎病（VW）是世界范围内重要的棉花（Gossypium hirsutum L.）病害之一。长期以来，使用抗性品种一直被认为是控制 VW 最实用、最有效的手段。陆地棉品种Prema具有较高的VW抗性，可用于培育抗VW的新品种。为了标记该品种中的抗性数量性状基因座 (QTL)，通过抗性 Prema 和易感品种 86-1 之间杂交的单粒种子培育了 180 个重组自交系群体。黄萎病抗性试验在接种混合菌株的人工病害苗圃和单独接种 VW 落叶菌株 V 991 和 V D8 的温室中进行，历时 2 年。通过对2010年和2011年人工病害苗圃调整病害反应数据进行复合区间作图分析，检出7个抗性QTL。其中，一致检测到一个主要的 VW 抗性 QTL，该 QTL 平均解释了 62.83% 的表型变异，对数范围为 23.37 至 26.73。该 QTL 被一致检测到并锚定在染色体 (chr.) D9 上。温室实验揭示了chr上存在5个显着的QTL。 A9、D3、D11 和 D9。我们的结果表明qVW-D9-1是主要的广谱VW抗性QTL。该QTL的鉴定将有助于棉花育种中VW抗性的标记辅助选择。
Verticillium wilt (VW) is one of the important damaging cotton (Gossypium hirsutum L.) diseases worldwide. The use of resistant cultivars has long been considered as the most practical and effective means of controlling VW. The upland cotton cultivar Prema has high VW resistance and is useful for breeding new cultivars resistant to VW. To tag the resistance quantitative trait loci (QTL) in this cultivar, a population of 180 recombinant inbred lines was developed via single seed decent from a cross between the resistant Prema and the susceptible cultivar 86-1. Verticillium wilt resistance trials were conducted in the artificial disease nursery inoculated with mixed isolates and greenhouse individually with VW defoliating isolates V 991 and V D8 over a 2-yr period. Based on composite interval mapping analysis of the 2010 and 2011 data sets of adjusted disease reaction in the artificial disease nursery, seven resistant QTL were detected. Among them, one major VW-resistant QTL, which explained 62.83% of the phenotypic variation on the average with logarithm of the odds scores ranging from 23.37 to 26.73, was detected consistently and anchored on chromosome (chr.) D9. Greenhouse experiments revealed the existence of five significant QTL on chr. A9, D3, D11, and D9. Our results showed that qVW-D9-1 is a major broad-spectrum VW resistance QTL. Identification of this QTL will facilitate the marker-assisted selection of VW resistance in cotton breeding.