RBM19 is essential for preimplantation development in the mouse.
RBM19 is essential for preimplantation development in the mouse.
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DOI：
10.1186/1471-213x-8-115
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发表时间：
2008-12-16
影响因子：
--
通讯作者：
Mayer AN
中科院分区：
文献类型：
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作者：
Zhang J;Tomasini AJ;Mayer AN
RNA结合基序蛋白19（RBM 19，NCBI登录号NP_083038）是含有6个保守RNA识别基序的保守核仁蛋白。它的生化功能是处理核糖体生物合成的rRNA，并已被证明在斑马鱼消化器官发育中发挥作用。在这里，我们分析了RBM 19在小鼠胚胎发育过程中的作用，通过基因陷阱插入产生小鼠含有突变的Rbm 19基因座。纯合子突变胚胎未能发展超过桑椹胚阶段，显示有缺陷的核形成，激活凋亡，P53靶基因的上调。RBM 19的一个独特特征是其定位于桑椹胚阶段胚胎的细胞质，而大多数其他核仁蛋白定位于核仁前体（NPB）。Rbm 19突变体胚胎中的核仁仍然不成熟，但它们可以进行rRNA合成。发育停滞的时间发生在内细胞团标志物OCT 3/4和NANOG的表达之后，但在滋养外胚层的特化之前，如CDX 2表达所反映的。这些数据表明，RBM 19是必不可少的植入前的发展，突出了在这个关键的发展阶段从头核仁的重要性。
RNA-binding motif protein 19 (RBM19, NCBI Accession # NP_083038) is a conserved nucleolar protein containing 6 conserved RNA recognition motifs. Its biochemical function is to process rRNA for ribosome biogenesis, and it has been shown to play a role in digestive organ development in zebrafish. Here we analyzed the role of RBM19 during mouse embryonic development by generating mice containing a mutation in the Rbm19 locus via gene-trap insertion. Homozygous mutant embryos failed to develop beyond the morula stage, showing defective nucleologenesis, activation of apoptosis, and upregulation of P53 target genes. A unique feature of RBM19 is its localization to the cytoplasm in morula stage-embryos, whereas most other nucleolar proteins are localized to the nucleolar precursor body (NPB). The nucleoli in the Rbm19 mutant embryos remain immature, yet they can carry out rRNA synthesis. The timing of developmental arrest occurs after expression of the inner cell mass markers OCT3/4 and NANOG, but prior to the specification of trophectoderm as reflected by CDX2 expression. The data indicate that RBM19 is essential for preimplantation development, highlighting the importance of de novo nucleologenesis during this critical developmental stage.