Protection against Trichinella spiralis in BALB/c mice via oral administration of recombinant Lactobacillus plantarum expressing murine interleukin-4
Protection against Trichinella spiralis in BALB/c mice via oral administration of recombinant Lactobacillus plantarum expressing murine interleukin-4
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通过口服表达鼠白细胞介素 4 的重组植物乳杆菌来预防 BALB/c 小鼠体内的旋毛虫
DOI：
10.1016/j.vetpar.2020.109068
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发表时间：
2020
影响因子：
2.6
通讯作者：
Wang Chun-Feng
中科院分区：
文献类型：
--
作者：
Wang Dan;Gong Qing-Long;Huang Hai-Bin;Yang Wen-Tao;Shi Chun-Wei;Jiang Yan-Long;Wang Jian-Zhong;Kang Yuan-Huan;Zhao Quan;Yang Gui-Lian;Wang Chun-Feng
白细胞介素-4 （IL-4）是一种来自活化T淋巴细胞的免疫调节细胞因子，可调节th2型免疫反应。它在抗寄生虫感染中起重要作用。本研究利用植物乳杆菌nc8的pgsa锚定系统共表达小鼠IL-4，设计了重组质粒，通过植物乳杆菌nc8传递，对BALB/c小鼠的旋毛虫感染有较强的免疫原性保护作用。结果表明，口服接种NC8-pSIP409-pgsA-mIL-4的小鼠血清IgG1和黏膜分泌IgA （sIgA）水平均显著升高，Th2表型免疫应答上调。在NC8-pSIP409-pgsA-mIL-4免疫刺激小鼠中，感染后7天（dpi）成虫负荷减少29.9%，感染后28天肌肉幼虫负荷减少83.3%。NC8-pSIP409-pgsA-mIL-4组小鼠的体重减轻和病理改变也有所改善。综上所述，NC8-pSIP409-pgsA-mIL-4可提高肠道黏膜免疫功能，促进肠内旋虫感染小鼠成虫的清除。该研究为开发新型旋毛虫病疫苗奠定了基础。
Interleukin-4 (IL-4), an immunomodulatory cytokine derived from activated T lymphocytes were shown to regulate Th2-type immune responses. It plays an important role in anti-parasitic infections. In this study, a recombinant plasmid was designed using murine IL-4 co-expressed withpgsAanchor system ofLactobacillus plantarumNC8 and was delivered by liveLactobacillus plantarumNC8, which exhibited an enhanced immunogenicity in protection of BALB/c mice from infection withTrichinella spiralis. The results showed that the levels of serum IgG1 and mucosal secretory IgA (sIgA) were both increased significantly in mice orally inoculated with NC8-pSIP409-pgsA-mIL-4, and the Th2 phenotype immune response was up-regulated. A 29.9 % reduction in adult worm burden at 7 days post-infection (dpi) and 83.3 % reduction in muscle larvae burden at 28 dpi were observed in immune-stimulated mice with NC8-pSIP409-pgsA-mIL-4. Moreover, weight loss and pathological changes were also improved in mice of NC8-pSIP409-pgsA-mIL-4 group. Taken together, it suggests that NC8-pSIP409-pgsA-mIL-4 could improve the intestinal mucosal immunity and promoted the elimination of the adult worm inTrichinella-infected mice. This study laid the foundation for the development of a novel vaccines against Trichinellosis.