The melanogenic effects and underlying mechanism of paeoniflorin in human melanocytes and vitiligo mice
The melanogenic effects and underlying mechanism of paeoniflorin in human melanocytes and vitiligo mice
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芍药苷对人黑素细胞和白癜风小鼠的黑色素生成作用及其机制
DOI：
10.1016/j.fitote.2019.104416
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发表时间：
2020-01-01
期刊：
影响因子：
3.4
通讯作者：
Li, Zhiming
中科院分区：
文献类型：
--
作者：
Hu, Murong;Chen, Cunguo;Li, Zhiming
白癜风是一种常见的色素脱失性疾病，病因不明，诊断简单，治疗困难，迫切需要新的有效治疗方法。芍药苷是从传统中药芍药根中提取的主要活性成分，其抗氧化应激等药理学特性已被证实参与白癜风的发生，但对黑素生成的影响尚不清楚。本研究对芍药苷的促黑素合成作用及其可能的作用机制进行了探讨。我们发现，芍药苷在10 μ g/ml的浓度处理显着增加黑色素含量和细胞内酪氨酸酶活性的人黑色素细胞，根据小眼相关转录因子（MITF），酪氨酸酶相关蛋白1（TRP-1）的蛋白水平的升高。此外，我们还研究了芍药苷促进cAMP反应元件结合（CREB）和细胞外信号调节激酶（ERK）的磷酸化，而不影响p38和c-Jun N-末端激酶（JNK）。这些结果表明芍药苷通过ERK/CREB途径对正常人黑素细胞具有协同作用，上调MITF和TRP-1，促进黑素合成。同时，芍药苷治疗白癜风小鼠的病理改变较轻，也证实了其治疗白癜风的潜力。提示芍药苷可能是临床上治疗白癜风的一种潜在药物。
Vitiligo is a common depigmentary disease characterized as diagnosis simplicity and cure difficulty in view of the ambiguity of etiology, thus novel and effective treatments are urgently needed. Paeoniflorin, the major active compound extracted from the root of Paeonia lactiflora Pall, a traditional Chinese medicine, has been validated pharmacological properties such as antioxidant stress, a theory participating in the occurrence of vitiligo, but the effect on melanogenesis is still unclear. In this study, melanosythesis effect of paeoniflorin and the potential mechanism were evaluated. We found that treatment with paeoniflorin at the concentration of 10 mu g/ml significantly increased melanin content and intracellular tyrosinase activity of human melanocytes, in accordance with the elevation of protein levels of microphthalmia-associated transcription factor (MITF), tyrosinase-related protein 1 (TRP-1). In addition, we also investigated that paeoniflorin promoted phosphorylation of cAMP-response element binding (CREB) and extracellular signal-regulated kinase (ERK) without affecting p38 and c-Jun N-terminal kinase (JNK). These results demonstrated that paeoniflorin had a synergistic effect on normal human melanocytes via ERK/CREB pathway with up-regulation of MITF and TRP-1, enhancing melanin synthesis. Meanwhile, the milder pathological changes in vitiligo mice treat with paeoniflorin also confirmed its potential in treating vitiligo. To sum up, we suggest that paeoniflorin may be a potential medicine of vitiligo treatment in clinical.