Human aquaporin-11 guarantees efficient transport of H2O2 across the endoplasmic reticulum membrane
Human aquaporin-11 guarantees efficient transport of H2O2 across the endoplasmic reticulum membrane
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DOI：
10.1016/j.redox.2019.101326
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发表时间：
2020-01-01
期刊：
影响因子：
11.4
通讯作者：
Medrano-Fernandez, Iria
中科院分区：
文献类型：
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作者：
Bestetti, Stefano;Galli, Mauro;Medrano-Fernandez, Iria
过氧化氢（H2O2）是细胞内重要的第二信使。为了达到其在胞质溶胶中的目标，H2O2必须穿过膜，这需要具有“过氧化物孔蛋白”活性的水通道蛋白（AQP）（AQP3，AQP8，AQP9）。在这里，我们利用不同的细胞器为目标的H2O2敏感的探针，以表明也AQP11有效地管道H2O2。与其他过氧化物酶不同，AQP11定位于内质网（ER）中，部分积聚在血管相关的ER膜（MAM）中。它的下调严重干扰H2O2通过ER的通量，但不通过线粒体或质膜。这些特性使AQP11成为ER氧化还原稳态和信号传导的潜在调节剂。
Hydrogen peroxide (H2O2) is an essential second intracellular messenger. To reach its targets in the cytosol, H2O2 must cross a membrane, a feat that requires aquaporins (AQP) endowed with 'peroxiporin' activity (AQP3, AQP8, AQP9). Here, we exploit different organelle-targeted H2O2-sensitive probes to show that also AQP11 efficiently conduits H2O2. Unlike other peroxiporins, AQP11 is localized in the endoplasmic reticulum (ER), accumulating partly in mitochondrial-associated ER membranes (MAM). Its downregulation severely perturbs the flux of H2O2 through the ER, but not through the mitochondrial or plasma membranes. These properties make AQP11 a potential regulator of ER redox homeostasis and signaling.