Timing and distribution of protein ingestion during prolonged recovery from resistance exercise alters myofibrillar protein synthesis
Timing and distribution of protein ingestion during prolonged recovery from resistance exercise alters myofibrillar protein synthesis
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DOI：
10.1113/jphysiol.2012.244897
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发表时间：
2013-05-01
影响因子：
5.5
通讯作者：
Coffey, Vernon G.
中科院分区：
文献类型：
--
作者：
Areta, Jose L.;Burke, Louise M.;Coffey, Vernon G.
蛋白质摄取的数量和时间是调节肌原纤维蛋白合成（MPS）的主要因素。然而，在12小时内，特定的摄入模式对MPS的影响是未知的。我们确定了在阻力运动后12小时恢复期间蛋白质喂养的不同分布如何影响骨骼肌的合成代谢反应。24名健康的训练有素的男性被分为三组（n= 8/组），并进行了一轮阻力运动，随后在12小时的恢复过程中摄入80克乳清蛋白，采用以下方案之一：每1.5小时8 x 10克（PULSE）；每3小时4 x 20 g（中间：INT）；或每6小时2 x 40 g （BOLUS）。分别于运动后1、4、6、7、12小时及休息时进行肌肉活检。评估静息和运动后MPS （l-[ring-13C6]苯丙氨酸）、肌肉mRNA丰度和细胞信号传导。在恢复的112小时内，所有摄入方案的MPS均高于休息（88148%,P < 0.02），但INT比PULSE和BOLUS诱导的MPS更高（3148%,P < 0.02）。总的来说，信号传导在磷酸化程度上表现为BOLUS>INT>PULSE层次。MuRF-1和SLC38A2 mRNA在BOLUS中表达差异。综上所述，每3小时摄入20克乳清蛋白，在全天刺激MPS方面优于PULSE或BOLUS喂养模式。这项研究提供了关于调节蛋白质摄入分布对骨骼肌合成代谢反应的影响的新信息，并有可能最大化阻力训练的结果，以达到峰值肌肉质量。
Quantity and timing of protein ingestion are major factors regulating myofibrillar protein synthesis (MPS). However, the effect of specific ingestion patterns on MPS throughout a 12 h period is unknown. We determined how different distributions of protein feeding during 12 h recovery after resistance exercise affects anabolic responses in skeletal muscle. Twenty-four healthy trained males were assigned to three groups (n= 8/group) and undertook a bout of resistance exercise followed by ingestion of 80 g of whey protein throughout 12 h recovery in one of the following protocols: 8 x 10 g every 1.5 h (PULSE); 4 x 20 g every 3 h (intermediate: INT); or 2 x 40 g every 6 h (BOLUS). Muscle biopsies were obtained at rest and after 1, 4, 6, 7 and 12 h post exercise. Resting and post-exercise MPS (l-[ring-13C6] phenylalanine), and muscle mRNA abundance and cell signalling were assessed. All ingestion protocols increased MPS above rest throughout 112 h recovery (88148%, P < 0.02), but INT elicited greater MPS than PULSE and BOLUS (3148%, P < 0.02). In general signalling showed a BOLUS>INT>PULSE hierarchy in magnitude of phosphorylation. MuRF-1 and SLC38A2 mRNA were differentially expressed with BOLUS. In conclusion, 20 g of whey protein consumed every 3 h was superior to either PULSE or BOLUS feeding patterns for stimulating MPS throughout the day. This study provides novel information on the effect of modulating the distribution of protein intake on anabolic responses in skeletal muscle and has the potential to maximize outcomes of resistance training for attaining peak muscle mass.