Fourier Transform Infrared Spectroscopy as a Method for Monitoring the Molecular Dynamics of Lymphocyte Activation
Fourier Transform Infrared Spectroscopy as a Method for Monitoring the Molecular Dynamics of Lymphocyte Activation
复制标题
傅里叶变换红外光谱作为监测淋巴细胞激活分子动力学的方法
DOI：
10.1366/0003702001949627
复制
发表时间：
2000
影响因子：
3.5
通讯作者：
D. McNaughton
中科院分区：
文献类型：
--
作者：
B. Wood;B. Tait;D. McNaughton
在本文中，我们报告了应用傅里叶变换红外（FT-IR）显微光谱技术监测淋巴细胞活化的分子动力学。用有丝分裂原植物血凝素-L 刺激的淋巴细胞的红外光谱在初始刺激后 15 分钟显示出在静息淋巴细胞中不明显的光谱特征。通过分析原始光谱的二阶导数并应用主成分分析 (PCA)，我们得出结论，第一个小时内观察到的主要光谱变化是由于总体 RNA 合成的增加造成的。在活化淋巴细胞的光谱中，1244、1080、1050、970、1160 和 1120 cm−1 处的 RNA 特征带随着时间的推移逐渐变得更加强烈。随着细胞分化为母细胞，这些变化的幅度随着时间的推移而增加。红外光谱对 RNA 部分的敏感性和该技术的快速性表明 FT-IR 光谱在组织相容性测试中未来可能发挥作用。
In this paper we report the application of Fourier transform infrared (FT-IR) microspectroscopy to monitor the molecular dynamics of lymphocyte activation. Infrared spectra of lymphocytes stimulated with the mitogen phytohaemagglutinin-L show spectral features 15 min after initial stimulation that are not apparent in resting lymphocytes. By analyzing the second-order derivatives of the raw spectra and applying principal components analysis (PCA), we conclude that the major spectral changes observed in the first hour result from an increase in overall RNA synthesis. Bands characteristic of RNA at 1244, 1080, 1050, 970, 1160, and 1120 cm−1 appear progressively more intense over time in the spectra of activated lymphocytes. The magnitude of these changes increases over time as the cell differentiates into a blast cell. The sensitivity of infrared spectroscopy to RNA moieties and the rapidity of the technique suggest a possible future role for FT-IR spectroscopy in histocompatibility testing.