Green fluorescent protein as a scaffold for intracellular presentation of peptides
Green fluorescent protein as a scaffold for intracellular presentation of peptides
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DOI：
10.1093/nar/26.2.623
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发表时间：
1998-01-15
影响因子：
14.9
通讯作者：
Kamb, A
中科院分区：
文献类型：
--
作者：
Abedi, MR;Caponigro, G;Kamb, A
肽适体为生物学过程提供探针，并为开发新的药物分子提供引物。此类适体类似于衍生自组合化学文库的化合物，其具有特异性结合或抑制活性。正如通常难以以定量方式确定组合化学文库的组成一样，确定体内展示的肽文库的质量和特征也是有问题的。为了帮助解决这些问题，我们采用了绿色荧光蛋白（GFP）作为展示构象受限肽的支架。GFP-肽文库允许分析细胞中的文库多样性和表达水平，并允许通过选择高荧光来富集文库中具有预定特征的序列，例如正确折叠蛋白的高表达。
Peptide aptamers provide probes for biological processes and adjuncts for development of novel pharmaceutical molecules, Such aptamers are analogous to compounds derived from combinatorial chemical libraries which have specific binding or inhibitory activities. Much as it is generally difficult to determine the composition of combinatorial chemical libraries in a quantitative manner, determining the quality and characteristics of peptide libraries displayed in vivo is problematical. To help address these issues we have adapted green fluorescent protein (GFP) as a scaffold for display of conformationally constrained peptides, The GFP-peptide libraries permit analysis of library diversity and expression levels in cells and allow enrichment of the libraries for sequences with predetermined characteristics, such as high expression of correctly folded protein, by selection for high fluorescence.