2 DISTINCT NONMUSCLE MYOSIN-HEAVY-CHAIN MESSENGER-RNAS ARE DIFFERENTIALLY EXPRESSED IN VARIOUS CHICKEN TISSUES - IDENTIFICATION OF A NOVEL GENE FAMILY OF VERTEBRATE NON-SARCOMERIC MYOSIN HEAVY-CHAINS
2 DISTINCT NONMUSCLE MYOSIN-HEAVY-CHAIN MESSENGER-RNAS ARE DIFFERENTIALLY EXPRESSED IN VARIOUS CHICKEN TISSUES - IDENTIFICATION OF A NOVEL GENE FAMILY OF VERTEBRATE NON-SARCOMERIC MYOSIN HEAVY-CHAINS
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DOI：
10.1111/j.1432-1033.1989.tb15057.x
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发表时间：
1989-10-01
期刊：
影响因子：
--
通讯作者：
MASAKI, T
中科院分区：
文献类型：
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作者：
KATSURAGAWA, Y;YANAGISAWA, M;MASAKI, T
从鸡成纤维细胞cDNA文库中，与鸡胗平滑肌MHC cDNA进行中等强度的交叉氢化，分离出两个不同的非肌球蛋白重链（non - muscle myosin heavy chain） cDNA克隆。这两种成纤维细胞MHC和砂囊MHC分别编码在鸡基因组的不同基因中。Northern blot分析显示，这两种非肌肉MHC mrna不仅在成纤维细胞中表达，而且在多种组织中表达，包括脑、肺、肾、脾、骨骼、心脏和平滑肌。然而，两种非肌肉MHC mrna的相对含量在不同组织中差异很大。非肌肉MHC编码的氨基酸序列彼此之间（81%的同源性）和与平滑肌MHC（81-84%）高度相似，但与脊椎动物骨骼肌MHC（38-41%）或原生动物非肌肉MHC（35-36%）的相似度要低得多。通过计算MHC序列之间的相似性得分，构建了MHC同工型的系统发育树。对该树的检查表明，脊椎动物的肌体（骨骼和心脏）MHC异构体编码在一个非常密切相关的多基因家族中，而脊椎动物的非肌体（平滑肌和非肌肉）MHC异构体定义了一个不同的、不太保守的MHC基因家族。
Two distinct cDNA clones for nonmuscle myosin heavy chain (MHC) were isolated from a chicken fibroblast cDNA library by cross-hydridization under a moderate stringency with chicken gizzard smooth muscle MHC cDNA. These two fibroblast MHC and the gizzard MHC are each encoded in different genes in the chicken genome. Northern blot analysis showed that both of the nonmuscle MHC mRNAs were expressed not only in fibroblasts but also in a variety of tissues including brain, lung, kidney, spleen, and skeletal, cardiac and smooth muscles. However, the relative contents of the two nonmuscle MHC mRNAs varied greatly among tissues. The encoded amino acid sequences of the nonmuscle MHCs were highly similar to each other (81% identity) and to the smooth muscle MHC (81-84%), but much less similar to vertebrate skeletal muscle MHCs (38-41%) or to protista nonmuscle MHCs (35-36%). A phylogenic tree of MHC isoforms was constructed by calculating the similarity scores between these MHC sequences. An examination of the tree showed that the vertebrate sarcomeric (skeletal and cardiac) MHC isoforms are encoded in a very closely related multigene family, and that the vertebrate non-sarcomeric (smooth muscle and nonmuscle) MHC isoforms define a distinct, less conserved MHC gene family.