Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics
Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics
复制标题
维持细胞活力和特性的最佳干细胞运输条件
DOI：
10.1007/s13770-018-0133-y
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发表时间：
2018
影响因子：
3.6
通讯作者：
T. Kwon
中科院分区：
文献类型：
--
作者：
N. Yu;S. Chun;Y. Ha;H. T. Kim;Dae Hwan Kim;Jeongshik Kim;J. Chung;J. N. Lee;P. Song;E. Yoo;B. Kim;T. Kwon
在细胞从实验台转运到患者的过程中，干细胞活力和特性的保存可以显著影响细胞治疗的成功。运输条件可考虑悬浮介质、时间、温度、细胞密度和容器类型等因素。为了确定最佳条件，分析了人羊水干细胞（AFSCs）在不同培养基(DMEM（H)、DMEM/F-12、K-SFM、RPMI 1640、α-MEM、DMEM(L)、PBS或生理盐水）、温度（4、22或37℃）、细胞密度（1 × 107个细胞悬浮在0.1、0.5、1.0或2.0 mL培养基中）和容器类型（塑料注射器或玻璃瓶）下的存活率。建立运输条件后，将AFSCs的干细胞特性与新鲜制备的细胞进行比较。在DMEM(H)中，细胞的存活率高于其他培养基。最佳转运温度为4℃，有效转运时间在12 h以内。细胞密度越低，存活率越高，考虑到临床方便，选择注射器作为转运容器。在干细胞特性的比较中，在既定条件下运输的细胞与新鲜制备的细胞表现出相似的效力。本研究结果可为优化干细胞转运条件提供基础。
The preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions. To establish optimal conditions, human amniotic fluid stem cells’ (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, α-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37 °C), cell density (1 × 107 cells were suspended in 0.1, 0.5, 1.0 or 2.0 mL of medium) and container type (plastic syringe or glass bottle). After establishing the transport conditions, stem cell characteristics of AFSCs were compared to freshly prepared cells. Cells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4 °C, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. In compare of stem cell characteristics, the transported cells with established conditions showed similar potency as the freshly prepared cells. Our findings can provide a foundation to optimization of conditions for stem cell transport.
影响因子：
4.5
作者：
Pollock, Kathryn;Sumstad, Darin;Kadidlo, Diane;McKenna, David H.;Hubel, Allison
通讯作者：
Hubel, Allison
影响因子：
3
作者：
Chen, Bo;Wright, Bernice;Connon, Che J.
通讯作者：
Connon, Che J.