Receipt of vaginal-cervical stimulation modifies synapsin content in limbic areas of the female rat.
Receipt of vaginal-cervical stimulation modifies synapsin content in limbic areas of the female rat.
复制标题
接受阴道宫颈刺激会改变雌性大鼠边缘区域的突触蛋白含量。
DOI：
10.1016/j.neuroscience.2008.02.048
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发表时间：
2008
期刊：
影响因子：
3.3
通讯作者：
Erskine,MS
中科院分区：
文献类型：
--
作者：
Oberlander,JG;Erskine,MS
雌性大鼠需要足够数量和模式的阴道-宫颈刺激来启动神经内分泌变化，这是成功将受精卵植入子宫所必需的。这些变化的特点是每天两次催乳素激增，持续10-12天。不育交配后，仍可观察到内分泌变化，称为假孕(PSP)。在怀孕或PSP之前启动这些变化所需的交配刺激具有神经表示，我们称之为插入助记。我们试图通过免疫标记突触前蛋白突触素来检查插入助记符的形成是否伴随着边缘区域突触数量或密度的变化。一组在发情前期骑自行车的雌性大鼠接受15或5次插管或坐骑--没有输精管结扎术的雄性插管；另一组时间匹配的对照组留在家中笼子里。所有雌性动物在90min或8h后灌流，取脑切片，杏仁核和海马区突触素免疫组织化学染色，共聚焦显微镜成像。结果发现，交配后90min，杏仁内侧背侧核(MePD)突触素点(相当于突触的免疫反应点)数量减少，突触素染色强度增强。在海马区的CA1区也观察到类似的点状减少，而在杏仁基底外侧核的强度增加。间隔插入对突触素的表达没有影响。在海马区观察到与接受阴道-颈部刺激无关的强度降低。所有这些效应在8h后都没有观察到。总之，这些结果提出了这样一种可能性，即在交配刺激后，MePD中的突触可能被修剪，导致路径特异性的稳定，这有助于与PSP的建立相关的内向助记。
Female rats require a sufficient amount and pattern of vaginal–cervical stimulation to initiate neuroendocrine changes required for the successful implantation of a fertilized ovum in the uterus. These changes are characterized by twice daily prolactin surges that last 10–12 days. Following a sterile mating, the endocrine changes are still observed, and are termed pseudopregnancy (PSP). The mating stimulation required to initiate these changes prior to pregnancy or PSP has a neural representation, which we have termed the intromission mnemonic. We sought to examine if the formation of the intromission mnemonic is accompanied by alterations in the number or density of synapses in limbic areas by immuno-labeling a pre-synaptic protein, synapsin. Groups of cycling female rats on proestrus day received either 15 or 5 intromissions or mounts-without intromissions from a vasectomized male; an additional time-matched control group was left in the home cage. All females were perfused after 90 min or 8 h. The brains were removed and sliced, and the amygdala and hippocampus immunostained for synapsin, then imaged by confocal microscopy. We found that 90 min after mating sufficient for PSP, the number of synapsin puncta (points of immunoreactivity equivalent to a synapse) was decreased and the intensity of the synapsin staining was increased in the posterodorsal medial amygdala (MePD). A similar reduction of puncta was observed in the CA1 region of the hippocampus, and an increase of intensity occurred in the basolateral amygdala. Spaced intromissions had no effect on synapsin expression anywhere examined. Intensity reductions unrelated to receipt of vaginal–cervical stimulation were observed in the hippocampus. None of these effects were observed after 8 h. Together, these results raise the possibility that synapses in the MePD may be pruned after mating stimulation, resulting in pathway-specific stabilization that contributes to the intromission mnemonic associated with the establishment of PSP.