CLONING AND EXPRESSION OF THE MOUSE PGK-1 GENE AND THE NUCLEOTIDE-SEQUENCE OF ITS PROMOTER
CLONING AND EXPRESSION OF THE MOUSE PGK-1 GENE AND THE NUCLEOTIDE-SEQUENCE OF ITS PROMOTER
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DOI：
10.1016/0378-1119(87)90214-9
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发表时间：
1987-01-01
期刊：
影响因子：
3.5
通讯作者：
MCBURNEY, MW
中科院分区：
文献类型：
--
作者：
ADRA, CN;BOER, PH;MCBURNEY, MW
我们报告的小鼠pgk-1基因编码的体细胞异构体的酶磷酸甘油酸激酶的克隆。该基因包含在X染色体的16 kb区域内，并被至少10个内含子中断。pgk-1基因的启动子区富含G和C核苷酸，含有5个拷贝的六脱氧核苷酸GGGCGG，这是Sp1转录因子的潜在结合位点，一个CCAAT序列，但没有TATA盒。该启动子在DNA介导的转染到哺乳动物细胞中后起作用。小鼠pgk-1基因的启动子与人pgk-1启动子同源。启动子中的许多保守基序可能表明这些序列在pgk-1基因表达中的重要作用。
We report the cloning of the mouse pgk-1 gene encoding the somatic cell isoform of the enzyme phosphoglycerate kinase. The gene is contained within a 16-kb region of the X chromosome and is interrupted by at least ten introns. The promoter region of the pgk-1 gene is rich in G and C nucleotides and contains five copies of the hexadeoxynucleotide, GGGCGG, the potential binding site for the Sp1 transcription factor, a CCAAT sequence, but no TATA box. This promoter functions following DNA-mediated transfection into mammalian cells. The promoter of the mouse pgk-1 gene is homologous to the human pgk-1 promoter. A number of conserved motifs in the promoter may indicate a significant role for these sequences in expression of the pgk-1 gene.