REGULATION BY SULFONATE GROUPS OF COMPLEMENT ACTIVATION INDUCED BY HYDROXYMETHYL GROUPS ON POLYSTYRENE SURFACES
REGULATION BY SULFONATE GROUPS OF COMPLEMENT ACTIVATION INDUCED BY HYDROXYMETHYL GROUPS ON POLYSTYRENE SURFACES
复制标题
DOI：
10.1016/0142-9612(93)90024-v
复制
发表时间：
1993-02-01
期刊：
影响因子：
14
通讯作者：
LABARRE, D
中科院分区：
文献类型：
--
作者：
MONTDARGENT, B;MAILLET, F;LABARRE, D
降低聚合物表面的补体激活能力对于提高其血液相容性非常重要。带有羟甲基（CH₂OH）基团的聚苯乙烯表面会激活补体替代途径。这种激活作用在很大程度上取决于基团的密度。带有磺酸根（SO₃⁻）基团的聚苯乙烯表面会吸附蛋白质，导致明显的激活。按相近比例带有这两种基团的聚苯乙烯表面在人血清中不会成为激活剂，这是因为替代途径的一种蛋白质发生了吸附，产生了保护作用，而带有羟基的聚合物表面与带有磺酸根的另一种聚合物表面在血清中混合时则没有这种保护作用。在存在纯化的替代途径蛋白质的情况下，通过C3b的沉积，在这些表面中的每一个上都可以产生C3转化酶活性，但它们被调节蛋白H和I灭活的敏感性取决于表面上存在的化学基团的类型以及在C3b沉积之前表面是否经过钝化处理。
Reducing the complement-activating capacity of a polymer surface is important in improving its blood compatibility. Polystyrene surfaces bearing hydroxymethyl (CH2OH) groups activate the alternative pathway of complement. This activation depends strongly on the density of the groups. Polystyrene surfaces bearing sulphonate (SO3-) groups adsorb proteins, resulting in an apparent activation. Polystyrene surfaces bearing both types of groups in close proportions are not activators in human serum, due to the adsorption of a protein of the alternative pathway, which has a protecting effect, not found when a polymer surface bearing hydroxyl groups is mixed in serum with another polymer surface bearing SO3- groups. In the presence of purified proteins of alternative pathway, C3 convertase activity can be created on each of these surfaces by deposition of C3b, but their susceptibility to inactivation by regulatory proteins H and I depends on the types of chemical groups present on the surface and whether the surfaces were passivated or not before C3b deposition.