Conformation-Directed Micelle-to-Vesicle Transition of Cholesterol Decorated Polypeptide Triggered by Oxidation
Conformation-Directed Micelle-to-Vesicle Transition of Cholesterol Decorated Polypeptide Triggered by Oxidation
复制标题
氧化引发的胆固醇修饰多肽的构象定向胶束到囊泡的转变
DOI：
10.1021/jacs.8b01873
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发表时间：
2018-05-30
影响因子：
15
通讯作者：
Fu, Qiang
中科院分区：
文献类型：
--
作者：
Liu, Hang;Wang, Rui;Fu, Qiang
合成多肽的分层自组装由于其具有类似蛋白质的结构，在纳米技术和生物医学领域具有巨大的应用潜力而受到越来越多的关注。然而，通过二级结构来控制聚合物纳米结构的形态和功能仍然是一个很大的未知领域。在这里，我们报道了一个不寻常的胶束到囊泡转化胆固醇修饰的聚（l-半胱氨酸）共聚物组装响应活性氧（ROS）。我们发现有趣的形态转变与从β -薄片到α -螺旋的构象改变有关，这为触发释放和细胞相互作用提供了一个有吸引力的“开关”开关。我们进一步证明了构象调节组装策略在体外和体内的有用性，以癌症治疗为模型。这项工作为多肽的折叠和分层组装提供了新的见解，并为生物传感、疾病治疗和诊断应用中的智能平台的开发提供了一种新方法。
Hierarchical self-assembly of synthetic polypeptides has attracted increasing interests due to its protein-mimetic structure and great potential in nanotechnology and biomedical applications. However, controlling the morphology and function of polymeric nanostructures via secondary structures remains largely unexplored. Here, we report an unusual micelle-to-vesicle transformation of cholesterol-decorated poly(L-cysteine) copolymer assemblies in response to reactive oxygen species (ROS). We found that the interesting morphological transition correlates with the alteration in conformations from beta-sheet to alpha-helix, which grants an attractive "on-off' switch for triggered release and cellular interaction. We further demonstrated the usefulness of the conformation-regulated assembly strategy both in vitro and in vivo, taking cancer treatment as a model. The work offers a new insight on the folding and hierarchical assembly of polypeptides and a novel approach for the development of smart platforms in biosensing, disease treatment, and diagnostic applications.