IDENTIFICATION AND CHARACTERIZATION OF RHIZOSPHERE-COMPETENT BACTERIA OF WHEAT
IDENTIFICATION AND CHARACTERIZATION OF RHIZOSPHERE-COMPETENT BACTERIA OF WHEAT
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DOI：
10.1128/aem.53.12.2793-2799.1987
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发表时间：
1987-12-01
影响因子：
4.4
通讯作者：
SANDS, DC
中科院分区：
文献类型：
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作者：
JUHNKE, ME;MATHRE, DE;SANDS, DC
为了获得能够适应根际的细菌，并将其用于生防剂的开发，用标准技术从小麦和大麦的根际和根面中分离到细菌。在这些菌株中，有60个菌株于1985年被选为春小麦种子接种剂进行田间试验。通过选择自发突变株来检测和监测田间分离株，对分离株进行了抗药性的遗传标记。种植43天后，突变菌株的根和根际土壤的平均log10cfu/mg(干重)在0到3.4之间。1986年，对20个突变菌株进行了重新检测。共有4个菌株未被检测到，但其余16个菌株的平均根部定殖值为log10 2.1CFU，种植32d后采样的范围为log10 0.9CFU至log10 3.2CFU。51天后平均定殖值降至log10 1.1 CFU。一些以前检测到的菌株在第二次采样中没有被检测到；其他一些菌株的根部定殖值与第一次采样中获得的值相似。根际细菌的突变菌株包括短小芽孢杆菌、枯草芽孢杆菌、荧光假单胞菌、链霉菌、嗜麦芽黄单胞菌和腐生棒状杆菌。来自不同属和种的混合菌株在种子和根上是相容的。
To obtain rhizosphere-competent bacteria which could subsequently be modified for the development of biological control agents, bacteria were isolated from the rhizosphere and rhizoplane of wheat and barley plants by standard techniques. Of these isolates, 60 were selected for field testing as spring wheat seed inoculants in 1985. Isolates were marked genetically for resistance to antibiotics via selection of spontaneous mutants to detect and monitor isolates in the field. Forty-three days after planting, the average log10 CFU/mg (dry weight) of roots and rhizosphere soil for the mutant isolates sampled ranged from 0 to 3.4. Twenty mutant isolates were retested in 1986. A total of 4 isolates were not detected, but the other 16 had an average root colonization value of log10 2.1 CFU and a range of log10 0.9 CFU to log10 3.2 CFU when sampled 32 days after planting. The average colonization value dropped to log10 1.1 CFU 51 days later. Some isolates detected previously were not detected in the second sampling; others had root colonization values similar to those obtained in the first sampling. Mutant isolates of rhizosphere bacteria included Bacillus pumilus, Bacillus subtilis, Pseudomonas fluorescens, Streptomyces spp., Xanthomonas maltophilia, and a saprophytic coryneform. Mixtures of isolates from different genera and species were compatible on seeds and roots.