Intracellular chloroplast photorelocation in the moss Physcomitrella patens is mediated by phytochrome as well as by a blue-light receptor
Intracellular chloroplast photorelocation in the moss Physcomitrella patens is mediated by phytochrome as well as by a blue-light receptor
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DOI：
10.1007/s004250050700
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发表时间：
2000-05-01
期刊：
影响因子：
4.3
通讯作者：
Wada, M
中科院分区：
文献类型：
--
作者：
Kadota, A;Sato, Y;Wada, M
研究了在红光下生长的小立碗藓原丝细胞中光诱导叶绿体的胞内迁移。当用偏振光或红光照射时，叶绿体在细胞中的分布取决于两种光质中的电矢量（E-矢量）的方向。当E-载体平行于横壁（即垂直于原丝体轴）时，叶绿体沿横壁沿着积累;而当E-载体垂直于横壁（即平行于原丝体轴）时，叶绿体不沿横壁沿着积累。当细胞的一部分被红色或蓝色微束照射时，根据所使用的注量率，叶绿体会在照射点处积累或避开照射点。0.1-18 W m（-2）红光和0.01-85.5 W m（-2）蓝光可诱导聚集反应（低通量率反应; LFR），而60 W m（-2）或更高红光和285 W m（-2）蓝光可诱导回避反应（高通量率反应; HFR）。红光诱导的LFR和HFR被同时的远红光背景照射所抵消，而蓝光诱导的LFR和HFR完全不受这种处理的影响。这些结果表明，第一次，二色性光敏色素，以及二色性蓝光受体，参与叶绿体搬迁运动在这些brandite细胞。此外，当红光生长的细胞在白色光下培养2 d时，光敏色素介导的反应而不是蓝光反应被揭示丢失。
The light-induced intracellular relocation of chloroplasts was examined in red-light-grown protonemal cells of the moss Physcomitrella patens. When irradiated with polarized red or blue light, chloroplast distribution in the cell depended upon the direction of the electrical vector (E-vector) in both light qualities. When the E-vector was parallel to the cross-wall (i.e. perpendicular to the protonemal axis), chloroplasts accumulated along the cross-wall; however, no accumulation along the cross-wall was observed when the vector was perpendicular to it (i.e. parallel to the protonemal axis). When a part of the cell was irradiated with a microbeam of red or blue light, chloroplasts accumulated at or avoided the illumination point depending on the fluence rate used. Red light of 0.1-18 W m(-2) and blue light of 0.01-85.5 W m(-2) induced an accumulation response (low-fluence-rate response; LFR), while an avoidance response thigh-fluence-rate response; HFR) was induced by red light of 60 W m(-2) or higher and by blue light of 285 W m(-2). The red-light-induced LFR and HFR were nullified by a simultaneous background irradiation of far-red light, whereas the blue-light-induced LFR and HFR were not affected at all by this treatment. These results show, for the first time, that dichroic phytochrome, as well as the dichroic blue-light receptor, is involved in the chloroplast relocation movement in these bryophyte cells. Further, the phytochrome-mediated responses but not the blue-light responses were revealed to be lost when red-light-grown cells were cultured under white light for 2 d.