Requirement of a CCGAC cis-acting element for cold induction of the BN115 gene from winter Brassica napus
Requirement of a CCGAC cis-acting element for cold induction of the BN115 gene from winter Brassica napus
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DOI：
10.1007/bf00049344
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发表时间：
1996-02-01
影响因子：
5.1
通讯作者：
Singh, J
中科院分区：
文献类型：
--
作者：
Jiang, C;Iu, B;Singh, J
对甘蓝型油菜冷诱导基因BN 115 5 ′端5 ′-近端区域的两个低温响应元件（LTREs）的核心五聚体CCGAC进行了突变。对所得到的突变的BN 115启动子-GUS融合体的瞬时表达的分析揭示了启动子的低温调节的损失。这表明CCGAC序列对BN 115基因中的低温响应至关重要。与此相反，两个G盒，CACGTG，在启动子的同一区域的LTRE之间交错的突变没有改变冷诱导基因的表达。用来自CaMV 35 S启动子的增强子替换BN 115启动子的可能的增强子区域导致低温诱导的GUS活性的几倍增加。
Mutation of the core pentamer, CCGAC, of two putative low temperature responsive elements (LTREs) in the 5'-proximal region of the winter Brassica napus cold-induced gene BN115 was carried out. Analyses of transient expression of the resultant mutated BN115 promoter-GUS fusions revealed the loss of low-temperature regulation by the promoter. This indicates that the CCGAC sequence is critical to the low-temperature response in the BN115 gene. In contrast, mutation of two G-boxes, CACGTG, staggered between the LTREs in the same region of the promoter did not alter cold-inducible gene expression. Replacement of a possible enhancer region of the BN115 promoter with the enhancer from the CaMV 35S promoter resulted in a several-fold increase in low temperature-induced GUS activity.