SOLUTE PERTURBATION OF PROTEIN FLUORESCENCE - QUENCHING OF TRYPTOPHYL FLUORESCENCE OF MODEL COMPOUNDS AND OF LYSOZYME BY IODIDE ION
SOLUTE PERTURBATION OF PROTEIN FLUORESCENCE - QUENCHING OF TRYPTOPHYL FLUORESCENCE OF MODEL COMPOUNDS AND OF LYSOZYME BY IODIDE ION
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DOI：
10.1021/bi00793a015
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发表时间：
1971-01-01
期刊：
影响因子：
2.9
通讯作者：
LEHRER, SS
中科院分区：
文献类型：
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作者：
LEHRER, SS
Sherwin S.Lehrer摘要：研究了碘对模型化合物和溶菌酶的色氨酸荧光的影响，以评价决定将碘作为蛋白质色氨酸侧链荧光选择性猝灭剂的因素。与模型化合物的结果表明，由于符合Stern-Volmer定律和猝灭常数与！用于吲哚-3-乙酰胺。利用可用寿命数据从测量的猝灭常数计算出的双分子速率常数K3对于不带电荷、带正电和带负电的双分子速率常数等于、大于或小于4-6×109TVr1秒~9。
Sherwin S. Lehrer abstract: The effect of iodide on the tryptophyl fluorescence of model compounds and of lysozyme was studied in order to evaluate the factors that determine the use of iodide as a selec-tive quencher of thefluorescence of tryptophyl side chains of proteins exposed to solvent. The results with the model com-pounds indicate the involvement of a collisional quenching mechanism due to the agreement with the Stern-Volmer law and the proportionality of the quenching constant with! for indole-3-acetamide. Bimolecular rate constants, k3, calculated from measured quenching constants using available lifetime data are equal to, greater than, or less than 4-6 X 109 tvr1 sec~ 9 for uncharged, positively charged, and nega-