Comparison of Self-Collected and Physician-Collected Vaginal Swabs for Microbiome Analysis
Comparison of Self-Collected and Physician-Collected Vaginal Swabs for Microbiome Analysis
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DOI：
10.1128/jcm.01710-09
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发表时间：
2010-05-01
影响因子：
9.4
通讯作者：
Ravel, Jacques
中科院分区：
文献类型：
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作者：
Forney, Larry J.;Gajer, Pawel;Ravel, Jacques
据我们所知，没有数据可获得的微生物种类组成和丰度取样的阴道拭子是否与临床医生收集的拭子相比较。20名健康女性在常规妇科就诊期间被招募到这项研究中。符合条件的女性年龄在18到40岁之间，月经周期正常。参与者使用标准化的程序自行收集阴道拭子，然后由医生检查，医生从阴道中部的侧壁收集另外五个拭子。本研究采用末端限制性内切片段长度多态性和16S rRNA基因序列分析，对自采拭子和3份医生获得的拭子进行分析和比较。阴道微生物群落的T-RFLP和16S rRNA基因序列数据集的比较统计分析显示，自采集阴道拭子样本的微生物多样性与医生采集阴道中部拭子样本相同。这些发现使得对阴道微生物组进行大规模的实地研究成为可能。
To our knowledge, no data are available on whether the microbial species composition and abundance sampled with self-collected vaginal swabs are comparable to those of swabs collected by clinicians. Twenty healthy women were recruited to the study during a routine gynecological visit. Eligible women were between 18 and 40 years old with regular menstrual cycles. Participants self-collected a vaginal swab using a standardized protocol and then were examined by a physician, who collected an additional five swabs from the lateral wall of the mid-vagina. In this study, the self-collected and three physician-obtained swabs were analyzed and compared using terminal restriction fragment length polymorphism and sequence analyses of the 16S rRNA genes. Vaginal microbial community comparative statistical analyses of both T-RFLP and 16S rRNA gene sequence datasets revealed that self-collected vaginal swabs sampled the same microbial diversity as physician collected swabs of the mid-vagina. These findings enable large-scale, field-based studies of the vaginal microbiome.